header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180622-33
Missinato et al., 2018 - Dusp6 attenuates Ras/MAPK signaling to limit zebrafish heart regeneration. Development (Cambridge, England)   145(5) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Fig. 7

EGF receptor inhibition in dusp6 mutants dampens cardiac regeneration. (A) Q-PCR analysis of errfi1b at 0 and 3 dpa. After amputation, dusp6 mutant hearts show higher errfi1b expression compared with WT. Data represent six independent replicates. (B-E) Hearts at 7 dpa injected for 6 days with AG1478 (C,E), or DMSO vehicle (B,D) and stained for Mef2c and Pcna to determine cardiomyocyte proliferation. Arrowheads indicate proliferating cardiomyocytes. For WT: DMSO, n=36; 10 µM and 25 µM AG1478, n=17 and n=14, respectively. For dusp6pt30a/pt30a: DMSO, n=31; 10 µM and 25 µM AG1478, n=19 and n=13, respectively. (F) Quantification of cardiomyocyte proliferation in WT and dusp6 mutant hearts after injection of AG1478 or DMSO. (G-J) Tg(fli1a:EGFP)y1 hearts at 8 dpa injected with AG1478 (H,J) or DMSO (G,I). MHC (red) marks the resection plane. AG1478 treatment blocked angiogenesis in WT hearts, but not in dusp6 mutant hearts. Dashed line demarcates resection plane. n=17 for DMSO in WT and dusp6pt30a/pt30a; n=7 for 25 µM AG1478 in WT and dusp6pt30a/pt30a. (K) Quantification of new vessels formed at 8 dpa. (L) Quantification of cardiomyocyte proliferation in erbb2st61/+ hearts at 7 dpa, injected with BCI215 (n=13) or DMSO (n=14) as compared with sibling WT hearts (DMSO, n=17). erbb2st61/+ hearts have a diminished cardiomyocyte proliferation index compared with WT hearts, and this is rescued by BCI215. (A,F,K,L) ****P<0.0001, ***P<0.001, *P<0.05; ns, not significant; one-way ANOVA. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
errfi1b WT standard conditions Adult heart ISH
WT resection: cardiac ventricle Adult heart ISH
dusp6pt30a/pt30a standard conditions Adult heart ISH
dusp6pt30a/pt30a resection: cardiac ventricle Adult heart ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mef2 IHC WT chemical treatment by injection: tyrphostin AG 1478, resection: cardiac ventricle Adult cardiac muscle cell
IHC WT resection: cardiac ventricle Adult cardiac muscle cell
IHC dusp6pt30a/pt30a chemical treatment by injection: tyrphostin AG 1478, resection: cardiac ventricle Adult cardiac muscle cell
IHC dusp6pt30a/pt30a resection: cardiac ventricle Adult cardiac muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by injection: tyrphostin AG 1478, resection: cardiac ventricle Adult cardiac muscle cell proliferation decreased process quality, abnormal
dusp6pt30a/pt30a chemical treatment by injection: tyrphostin AG 1478, resection: cardiac ventricle Adult cardiac muscle cell proliferation process quality, normal
dusp6pt30a/pt30a resection: cardiac ventricle Adult cardiac muscle cell proliferation increased process quality, abnormal
Adult heart errfi1b expression increased amount, abnormal
dusp6pt30a/pt30a ; y1Tg chemical treatment by injection: tyrphostin AG 1478, resection: cardiac ventricle Adult regenerating tissue angiogenesis process quality, normal
erbb2st61/+ resection: cardiac ventricle Adult cardiac muscle cell proliferation decreased process quality, abnormal
y1Tg chemical treatment by injection: tyrphostin AG 1478, resection: cardiac ventricle Adult regenerating tissue angiogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development