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ZFIN ID: ZDB-FIG-180622-31
Missinato et al., 2018 - Dusp6 attenuates Ras/MAPK signaling to limit zebrafish heart regeneration. Development (Cambridge, England)   145(5) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Fig. 5

Chemical inhibition of Dusp6 increases cardiomyocyte proliferation and angiogenesis and reduces fibrosis after cardiac injury. (A-C) Adult zebrafish hearts at 7 dpa, injected for 6 days with DMSO (n=16) (A), 0.5 mg/kg BCI (n=13) (B) or 0.5 mg/kg BCI215 (n=12) (C) and stained for Mef2c and Pcna. (D) Quantification of proliferating cardiomyocytes at 7 dpa. BCI and BCI215 increased cardiomyocyte proliferation compared with DMSO vehicle. (E-G) Tg(fli1a:EGFP)y1 hearts at 8 dpa injected for 6 days with BCI (n=16) (F), BCI215 (n=19) (G) or DMSO (n=14) (E) and stained for Mef2c. (H) Quantification of new vessels formed inside the clot area of hearts at 8 dpa. (I-K) Sections of hearts at 25 dpa stained with AFOG to visualize the scar. Intact cardiac muscle stains orange-brown, fibrin stains red and collagen blue. Fish injected with BCI (n=12) (J) and BCI215 (n=18) (K) resolved the injury faster than DMSO-injected fish (n=14) (I). (L) Quantification of clot areas at 25 dpa. (D,H,L) ****P<0.0001, ***P<0.001, **P<0.01, *P<0.05, one-way ANOVA. Scale bars: 100 µm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mef2 IHC WT chemical treatment by injection: 2-Benzylidene-3-(cyclohexylamino)-3H-inden-1-one chloride, resection: cardiac ventricle Adult cardiac muscle cell
IHC WT chemical treatment by injection: BCI-215, resection: cardiac ventricle Adult cardiac muscle cell
IHC WT resection: cardiac ventricle Adult cardiac muscle cell
IHC y1Tg chemical treatment by injection: 2-Benzylidene-3-(cyclohexylamino)-3H-inden-1-one chloride, resection: cardiac ventricle Adult cardiac muscle cell
IHC y1Tg chemical treatment by injection: BCI-215, resection: cardiac ventricle Adult cardiac muscle cell
IHC y1Tg resection: cardiac ventricle Adult cardiac muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by injection: 2-Benzylidene-3-(cyclohexylamino)-3H-inden-1-one chloride, resection: cardiac ventricle Adult cardiac muscle cell increased amount, abnormal
Adult cardiac muscle cell proliferation increased process quality, abnormal
Adult heart structure, ameliorated
WT chemical treatment by injection: BCI-215, resection: cardiac ventricle Adult cardiac muscle cell increased amount, abnormal
Adult cardiac muscle cell proliferation increased process quality, abnormal
Adult heart structure, ameliorated
WT resection: cardiac ventricle Adult heart scarred, abnormal
y1Tg chemical treatment by injection: 2-Benzylidene-3-(cyclohexylamino)-3H-inden-1-one chloride, resection: cardiac ventricle Adult cardiac muscle tissue regeneration increased rate, abnormal
Adult regenerating tissue angiogenesis increased process quality, abnormal
y1Tg chemical treatment by injection: BCI-215, resection: cardiac ventricle Adult cardiac muscle tissue regeneration increased rate, abnormal
Adult regenerating tissue angiogenesis increased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development