header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180608-69
Eve et al., 2017 - Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo. Wellcome open research   2:111 Full text @ Wellcome Open Res
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 2

Lamc3 knockdown embryos do not develop the parachordal chain (PAC).

(AB) Lateral view of the zebrafish trunk stained using anti-LAMC3 antibody shows reduction in Lamc3 protein at 50 hpf in knockdown embryos (E, n=10) compared to standard controls (D, n=11). (CI) Vasculature of 48 hpf Tg(fli1a:egfp) embryos. (C) Embryos injected with standard control MO develop the PAC. (D) Lamc3 knockdown embryos do not develop the PAC. (E) An enlarged image of the trunk vasculature in control embryos the PAC is indicated by a white arrowhead. (F) An enlarged image of the trunk vasculature in Lamc3 SBMO injected embryos. (G) The parachordal chain in control embryos at 72 hpf (white arrowhead). (H) By 72 hpf Lamc3 embryos have still not developed a parachordal chain. (I) Quantification of all ISVs with a PAC in Tg(fli1a:egfp) uninjected (white, n=545), control (green, n=462), Lamc3 SBMO knockdown (magenta, n=1133) and Lamc3 TBMO knockdown embryos (blue, n=880) at 48 hpf and 72 hpf, represent

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s896Tg ; hu4624Tg + MO1-lamc3 + MO4-tp53 standard conditions Protruding-mouth intersegmental artery amount, normal
Protruding-mouth intersegmental vein amount, normal
Protruding-mouth posterior cardinal vein sprouting angiogenesis process quality, normal
sd2Tg; y1Tg + MO1-lamc3 + MO4-tp53 standard conditions Protruding-mouth intersegmental vessel parachordal vessel decreased amount, abnormal
Protruding-mouth parachordal vessel aplastic, abnormal
sd2Tg; y1Tg + MO2-lamc3 + MO4-tp53 standard conditions Protruding-mouth intersegmental vessel parachordal vessel decreased amount, abnormal
Protruding-mouth parachordal vessel aplastic, abnormal
y1Tg ; nim5Tg + MO1-lamc3 + MO4-tp53 standard conditions Day 5 thoracic duct absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Wellcome open research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Wellcome Open Res