ZFIN ID: ZDB-FIG-180608-68
Eve et al., 2017 - Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo. Wellcome open research   2:111 Full text @ Wellcome Open Res
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 1

Lamc3 expression during zebrafish development.

(A) MUSCLE-aligned protein “fingerprints” of zebrafish (Danio rerio, Dre), human (Homo sapiens, Hsa), and mouse (Mus musculus, Mmu) LAMC1, LAMC2, and LAMC3 amino acid sequences with RasMol colouring. (B) Unrooted RaxML phylogenetic tree (JTT+Gamma model) of Nematostella vectensis (purple, Nve) Lamc1-like and human (red, Hsa), mouse (orange, Mmu), and zebrafish (blue, Dre) LAMC1, LAMC2, and LAMC3 proteins. Branch labels denote ML bootstrap values (100). (C) Aligned human, mouse, and zebrafish amino acid sequence of the laminin γ1 and γ3 C-terminal tails show that the loss of the essential glutamic acid residue in γ3 is conserved across vertebrates. (D) qRT-PCR shows lamc3 is not significantly enriched in gfp+ endothelial cells (1.85E-05±5.49E-06, n=3) compared to GFP- cells (6.28E-06±1.48E-06, n=4) sorted from 26 hpf Tg(fli1a:egfp) embryos. Expression is relative to ef1α expression and normalised to GFP+ population (100%). All val

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lamc3 Ab1-lamc3 AB standard conditions Prim-5 floor plate IHC
Prim-5 head ISH
Ab1-lamc3 Prim-5 horizontal myoseptum IHC
Ab1-lamc3 Prim-5 Not Detected trunk vasculature IHC
Prim-5 vasculature ISH
Ab1-lamc3 Long-pec axial vasculature IHC
Long-pec fin bud ISH
Ab1-lamc3 Long-pec floor plate IHC
Long-pec head ISH
Ab1-lamc3 Long-pec horizontal myoseptum IHC
Long-pec horizontal myoseptum ISH
Long-pec vasculature ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Wellcome open research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Wellcome Open Res