header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180523-8
Fu et al., 2018 - Novel Animal Model of Crumbs-Dependent Progressive Retinal Degeneration That Targets Specific Cone Subtypes. Investigative ophthalmology & visual science   59:505-518 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 8

Secreted Crb2b-sfEX induces apoptosis in cones. Retinal flat-mount preparations double-immunolabeled with anti-Crb2a (green) and the apoptotic marker, anti-active caspase 3 (magenta). (AA''', BB''') Cells labeled with anti-active caspase 3 were in the photoreceptor layer, as indicated by anti-Crb2a label. (AA'') and (BB'') represent 2D planar views, and (A''') and (B''') include 3D-reconstructed “cut views” (in the xz and xy planes) at the level of the OLM, indicated by the white cross hairs, demonstrating that apoptotic and photoreceptor-specific labels are in the photoreceptor layer. (C–C''') Active caspase 3–labeled photoreceptors were not observed in WT retinas.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crb2a WT standard conditions Adult retinal cone cell IHC
pt108bTg standard conditions Adult retinal cone cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-casp3 IHC WT standard conditions Adult retinal cone cell
IHC pt108bTg standard conditions Adult retinal cone cell
Phenotype Details
Fish Conditions Stage Phenotype
pt108bTg standard conditions Adult retinal cone cell ab1-casp3 labeling increased distribution, abnormal
Adult retinal cone cell apoptotic process increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.