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ZFIN ID: ZDB-FIG-180523-6
Fu et al., 2018 - Novel Animal Model of Crumbs-Dependent Progressive Retinal Degeneration That Targets Specific Cone Subtypes. Investigative ophthalmology & visual science   59:505-518 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

Secreted Crb2b-sfEX affects photoreceptor patterning and maintenance. (A) The expression patterns of Crb genes are highlighted with different colors superimposed on a transmission electron microscopic image of a tangential section through the photoreceptor layer. The Crb2b-sfEX transgene in pt108b is mostly expressed in blue cones; endogenous crb2a is expressed in all photoreceptors and Müller cells; endogenous crb2b is expressed selectively in RGB cones. (B) In flat-mount retinal preparations at 4 mpf, ZO-1 immunolabeled apical profiles of photoreceptors in pt108b retina form a mosaic pattern, but the regular organization is degraded by 18 mpf. (C, C') JB4-Feulgen staining of retinal cross sections from wild-type (WT) (C) and pt108 (C') illustrates severe reduction of RGB cones (arrows) in pt108b retina at 27 mpf, although rods and UV cone nuclei (arrowheads) are maintained. Dashed lines, OLM. (D, D') In WT retina (D) at 27 mpf Zpr1, immunolabeled green and red cones have elongated nuclei (arrows), but in pt108 (D'), the nuclei of the few remaining Zpr1-labeled cones are less elongated (arrow). UV opsin immunoreactivity (asterisks) verified the UV cone identity of nuclei (arrowheads) that are abnormally positioned apical to the OLM. DAPI (4′,6-diamidino-2-phenylindole), nuclear stain. Dashed lines, OLM. (E, E') ZO1 immunostaining (arrows) revealed the OLM, which is positioned apical to the UV cone nuclei in WT (E, arrowheads) but basal to UV cone nuclei in pt108b (E', arrowheads). UV cone outer segments were visualized by UV opsin immunostaining (asterisks).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-opn1sw1 IHC WT standard conditions Adult retina UV sensitive photoreceptor cell
IHC pt108bTg standard conditions Adult retina UV sensitive photoreceptor cell
Ab1-tjp1 IHC WT standard conditions Adult retina green sensitive photoreceptor cell
IHC Adult retina red sensitive photoreceptor cell
IHC pt108bTg standard conditions Adult retina green sensitive photoreceptor cell
IHC Adult retina red sensitive photoreceptor cell
Phenotype Details
Fish Conditions Stage Phenotype
pt108bTg standard conditions Adult retina green sensitive photoreceptor cell ab1-tjp1 labeling decreased amount, abnormal
Adult retina red sensitive photoreceptor cell ab1-tjp1 labeling decreased amount, abnormal
Adult retina UV sensitive photoreceptor cell displaced, abnormal
Adult retina UV sensitive photoreceptor cell ab1-opn1sw1 labeling mislocalised, abnormal
Adult retinal cone cell spatial pattern, abnormal
Adult retinal cone cell development process quality, normal
Adult retinal cone cell nucleus morphology, abnormal
Adult retinal neural layer spatial pattern, normal
Adult retinal rod cell nucleus morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.