ZFIN ID: ZDB-FIG-180522-38
Fadeev et al., 2018 - ALKALs are in vivo ligands for ALK family receptor tyrosine kinases in the neural crest and derived cells. Proceedings of the National Academy of Sciences of the United States of America   115(4):E630-E638 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Adult

Fig. 4

DrAlkals affect iridophore development via DrLtk. (A) Ectopic expression of DrAlkal2a does not rescue loss of iridophores in 4 dpf transheterozygous ltk knockout zebrafish larvae (2 bp insertion/22 bp insertion, n = 9/9), whereas in heterozygous or wild-type siblings and ltkj9s1 larvae, it leads to overproduction of iridophores (n = 29/29 and 34/35). Overexpression of DrAlkal2a in ltkmne mutant slightly enhances the phenotype. (B) Treatment with lorlatinib results in diminished iridophore numbers in 4 dpf larvae. (C) Mosaic overexpression of DrAlkal2a produces patches of supernumerary iridophores in wild-type and ltkmne adults. Overexpression of DrAlkal2a in ltkmne mutants enhances production of supernumerary iridophores compared with uninjected ltkmne. (D) Zebrafish alkal mutants display defects in iridophore development. alkal1ko mutants have reduced iridophores in eyes and operculum, and removal of both alkal1and alkal2b results in a complete loss of eye iridophores. alkal2ako mutants show the strongest phenotype, with reduced numbers of iridophores in the trunk, especially anteriorly. This phenotype displays increased penetrance in alkal2a;alkal2b double mutants. (E) Triple mutant for all alkal genes is embryonic lethal and displays total loss of iridophores, similar to ltk transheterozygous knockout.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
alkal1t613A/+ ; alkal2at614A/t614A ; alkal2bt615B/+ standard conditions Adult iridophore decreased amount, abnormal
Adult melanophore stripe irregular spatial pattern, abnormal
Adult melanophore stripe morphology, abnormal
alkal1t613A/t613A standard conditions Adult opercular flap has fewer parts of type iridophore, abnormal
Adult opercular flap iridophore decreased amount, abnormal
alkal1t613A/t613A ; alkal2at614A/t614A standard conditions Adult opercular flap has fewer parts of type iridophore, abnormal
Adult opercular flap iridophore decreased amount, abnormal
alkal1t613A/t613A ; alkal2bt615B/t615B standard conditions Adult eye has fewer parts of type iridophore, abnormal
Adult eye iridophore decreased amount, abnormal
Adult opercular flap has fewer parts of type iridophore, abnormal
Adult opercular flap iridophore decreased amount, abnormal
alkal2at614A/t614A standard conditions Adult iridophore decreased amount, abnormal
Adult melanophore stripe irregular spatial pattern, abnormal
Adult melanophore stripe morphology, abnormal
alkal2at614A/t614A ; alkal1t613A/t613A ; alkal2bt615B/t615B standard conditions Protruding-mouth whole organism dead, abnormal
Protruding-mouth whole organism lacks all parts of type iridophore, abnormal
alkal2at614A/t614A ; alkal2bt615B/+ standard conditions Adult iridophore decreased amount, abnormal
Adult melanophore stripe irregular spatial pattern, abnormal
Adult melanophore stripe morphology, abnormal
alkal2at614A/t614A ; alkal2bt615B/+ ; alkal1t613A/t613A standard conditions Adult iridophore decreased amount, abnormal
Adult melanophore stripe irregular spatial pattern, abnormal
Adult melanophore stripe morphology, abnormal
alkal2bt615B/t615B standard conditions Adult melanophore stripe morphology, normal
alkal2bt615B/t615B ; alkal1t613A/t613A ; alkal2at614A/+ standard conditions Adult eye has fewer parts of type iridophore, abnormal
Adult eye iridophore decreased amount, abnormal
Adult opercular flap has fewer parts of type iridophore, abnormal
Adult opercular flap iridophore decreased amount, abnormal
ltkdtwc3/dtwc3 standard conditions Protruding-mouth whole organism lacks all parts of type iridophore, abnormal
ltkj9s1/j9s1 standard conditions Protruding-mouth whole organism lacks all parts of type iridophore, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA