ZFIN ID: ZDB-FIG-180522-37
Fadeev et al., 2018 - ALKALs are in vivo ligands for ALK family receptor tyrosine kinases in the neural crest and derived cells. Proceedings of the National Academy of Sciences of the United States of America   115(4):E630-E638 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 2

Expression of DrAlkals is sufficient for ectopic production of iridophores. (A) Schematic of ALKAL-expression constructs. AmpR, ampicillin resistance; Tol2, medaka Tol2 transposase recognition sequence. (B) alkal expression in 72 hpf albino larvae. Weak alkal1 signal can be observed in the head, swim bladder (B1), notochord (B2; green arrowheads), and iridophores in dorsal and ventral stripes (B2; blue arrowheads). alkal2a mRNA is detected in the head (B3), swim bladder (B4), notochord (B5; green arrowheads), and iridophore stripes (B5; blue arrowheads). alkal2b mRNA is detected in swim bladder, a row of cells at the ventral aspect of the head (B7; white arrowhead), bilateral clusters in the head (B6; red arrowheads), behind the eyes (B6; black arrowheads), notochord (B8; green arrowheads), and iridophore stripes (B8; blue arrowheads). ltk expression is visible in swim bladder (B9), eyes (B10), notochord (green arrowheads), and iridophores (blue arrowheads). Negative controls, corresponding sense probes. (C) Supernumerary iridophores were observed in 5 dpf larvae upon ectopic expression of indicated DrAlkals in F0-injected fish. ltkmne larvae are used as positive controls. (D) Distribution of phenotypes of C. Fisher exact probability test showed significant differences (P < 0.001) in phenotype distribution in the following comparisons: any Alkal overexpressing fish against uninjected control and Akal2a overexpressing fish against Alkal1 or Alkal2b overexpressing fish. No significant difference was found between Alkal1 and Alkal2b overexpressing fish (P = 0.2). (E) Mosaic overexpression of alkal2a produces patches of supernumerary iridophores in adults.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alkal1 slc45a2b4/b4 standard conditions Protruding-mouth eye ISH
Protruding-mouth iridophore ISH
Protruding-mouth notochord ISH
Protruding-mouth swim bladder ISH
alkal2a slc45a2b4/b4 standard conditions Protruding-mouth eye ISH
Protruding-mouth iridophore ISH
Protruding-mouth notochord ISH
Protruding-mouth swim bladder ISH
alkal2b slc45a2b4/b4 standard conditions Protruding-mouth head cell ISH
Protruding-mouth swim bladder ISH
ltk slc45a2b4/b4 standard conditions Protruding-mouth eye ISH
Protruding-mouth head ISH
Protruding-mouth iridophore ISH
Protruding-mouth swim bladder ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA