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ZDB-FIG-180424-28

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Uribe et al., 2017 - Retinoic acid temporally orchestrates colonization of the gut by vagal neural crest cells

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Fig. S1

Heat shock attenuation of RA signaling function leads to delayed colonization of the gut by enteric neural crest cells. (A-B) crestin whole-mount in situ hybridization in control heat shock (GFP^-) larvae (A) and heat shock dnRaraa-GFP⁺ larvae (B) at 48 hpf, arrowhead points to anterior foregut region. Dorsal views reveal that neural crest cell entry along the foregut is delayed (arrows) in dnRaraa-GFP⁺ larvae (B’), when compared with control larvae (A’). vg-vagal, fb-fin bud (C) Cartoon schematic to depict genotype and time course of heat shock attenuation experiments shown in panels D-G. (D-G) Live confocal images showing sox10:mRFP^-/+; hsp70:dnRaraa-GFP^-/+ larvae at 52 hpf to reveal enteric neural crest chain localization along the gut. Lateral views show that enteric neural crest reside within the foregut following heat shock induction of dnRaraa-GFP, while control heat shock enteric neural crest are located within the midgut at the same time, asterisks denote caudal end of the migratory chain. Scale bar in D and F, 100 microns; in E and G, 50 microns.

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Antibody Labeling

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Reprinted from Developmental Biology, 433(1), Uribe, R.A., Hong, S.S., Bronner, M.E., Retinoic acid temporally orchestrates colonization of the gut by vagal neural crest cells, 17-32, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.