ZFIN ID: ZDB-FIG-180202-17
Farwell et al., 2017 - Novel Heparin Receptor Transmembrane Protein 184a Regulates Angiogenesis in the Adult Zebrafish Caudal Fin. Frontiers in Physiology   8:671 Full text @ Front. Physiol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Adult

Fig. 1

Ontogenic and regenerating zebrafish fins express Tmem184a in the vasculature. Representative confocal microscopy images of cryogenic transverse sections of regenerating and ontogenic Tg(Fli1:EGFP) fins. All sections were stained with a GFP antibody to enhance EC fluorescence/visualization along with DAPI to easily identify fin rays. (A) Sections were stained for Tmem184a using a commercial antibody. (B) Sections were stained for Tmem184a using a zebrafish-specific antibody (ZF-CTD). Each image shows one individual fin ray. The arrowhead points to a mature artery, and the arrows mark mature veins. Scale bars = 50 μm. DAPI staining is blue, EC staining is green, and Tmem184a staining is red for all images. Sections from more than 10 individual fish were immunostained and analyzed for each combination of antibodies.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tmem184a Ab2-tmem184a y1Tg standard conditions Adult post-vent vasculature endothelial cell IHC
Ab2-tmem184a y1Tg amputation: caudal fin Adult post-vent vasculature endothelial cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tmem184a IHC y1Tg standard conditions Adult post-vent vasculature endothelial cell
IHC y1Tg amputation: caudal fin Adult post-vent vasculature endothelial cell
Ab5-GFP IHC y1Tg standard conditions Adult vasculature
IHC y1Tg amputation: caudal fin Adult vasculature
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Physiology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Physiol.