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ZIRC
ZFIN ID: ZDB-FIG-180112-13
Facchinello et al., 2017 - nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor. Scientific Reports   7:4371 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. S1

(A): qRT-PCR of gr mRNA in 5-dpf mutant larvae compared to control shows a statistically significant reduction of gr expression. Values represent the mean ± SEM. Asterisks indicate that expression levels are significantly different from the control: ***P < 0.001. Data were generated from four biological replicates. (B): Representative images of 5-dpf control gr+/+ and mutant gr-/- larvae after exposure to VBA stimulus. gr-/- mutants appear darker in comparison to control. (C): Representative gel image of PCR genotyping using genomic DNA from tail fins of adults born from a cross between gr heterozygotes. (D): Western blot of liver proteins from 8-month-old gr-/- and gr+/+ zebrafish showing disappearance of the protein band with respect to control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nr3c1 WT standard conditions Day 5 whole organism RTPCR
nr3c1ia30/ia30 standard conditions Day 5 whole organism RTPCR
nr3c1s357/s357 standard conditions Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nr3c1ia30/ia30 standard conditions Day 5 whole organism nr3c1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.