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General Information
ZFIN ID: ZDB-FIG-180112-13
Facchinello et al., 2017 - nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor. Scientific Reports   7:4371 Full text @ Sci. Rep.
Anatomical Term:
Stage: Day 5
Observed In:
Stage: Day 5

Fig. S1

(A): qRT-PCR of gr mRNA in 5-dpf mutant larvae compared to control shows a statistically significant reduction of gr expression. Values represent the mean ± SEM. Asterisks indicate that expression levels are significantly different from the control: ***P < 0.001. Data were generated from four biological replicates. (B): Representative images of 5-dpf control gr+/+ and mutant gr-/- larvae after exposure to VBA stimulus. gr-/- mutants appear darker in comparison to control. (C): Representative gel image of PCR genotyping using genomic DNA from tail fins of adults born from a cross between gr heterozygotes. (D): Western blot of liver proteins from 8-month-old gr-/- and gr+/+ zebrafish showing disappearance of the protein band with respect to control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nr3c1 WT standard conditions Day 5 whole organism RTPCR
nr3c1ia30/ia30 standard conditions Day 5 whole organism RTPCR
nr3c1s357/s357 standard conditions Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nr3c1ia30/ia30 standard conditions Day 5 whole organism nr3c1 expression decreased amount, abnormal
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.