ZFIN ID: ZDB-FIG-171229-3
Shainer et al., 2017 - Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish. Scientific Reports   7:44777 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Days 7-13

Fig. 3

AgRP2 preoptic neurons project towards the pituitary and terminate at the pituitary vasculature.

(a) A cross between agrp2:mCherry and Tg(oxt:EGFP)wz01 shows that AgRP2 neurons (magenta) are localized to the preoptic area, adjacent to oxytocin neurons (green), dorsal view of a 5-dpf larva head. (b) Higher-gain imaging of a 5-dpf agrp2:mCherry larva reveals projections of the AgRP2 preoptic neurons towards the pituitary (arrows). (c) Pituitary of a 12-dpf agrp2:mCherry, Tg(pomc:EGFP)zf44 larva, showing AgRP2 projections (magenta) terminating at the adenohypophysis, in close proximity to anterior POMC neurons (green). (d) Pituitary of a 12-dpf agrp2:mCherry, Tg(oxt:EGFP)wz01 larva, showing that projections from AgRP2 (magenta) and oxytocin (green) neurons share the same axonal tracts (arrows) but terminate at different locations within the pituitary: AgRP2 axons terminate at an anterior region whereas oxytocin axons terminate at a posterior region. (e) Pituitary of a 7-dpf agrp2:mCherry, Tg(UAS:SYP-EGFP)biu5 larva expressing synaptophysin (SYP)-EGFP fusion protein and mCherry in AgRP2 neurons. SYP-EGFP protein aggregates in pre-synaptic vesicles at the adenohypophysial AgRP2 terminals. (f) Z-stack projection of a 12-dpf agrp2:mCherry, Tg(kdrl:EGFP)s843 larval pituitary, showing that AgRP2 preoptic projections (magenta) form an interface with the pituitary vasculature (green). (gg”) show higher magnification of (f). (g) A single focal plane of the pituitary vasculature. (g’) AgRP2 terminals form an arc along the pituitary artery. (g”) Merged image of (g) and (g’). The observed overlap between AgRP2 terminals and the pituitary artery demonstrates a neurovascular interface of AgRP2 terminals with the pituitary vasculature. Anterior to top. AH, adenohypophysis; NH, neurohypophysis; Pit, pituitary; POA, preoptic area. Scale bar, 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP tlv05Tg ; biu5Tg ; c264Tg control Days 7-13 adenohypophysis presynapse IFL
tlv05Tg ; c264Tg ; s843Tg control Days 7-13 hypophysis vascular endothelium IFL
tlv05Tg ; wz01Tg ; c264Tg control Day 5 preoptic area IFL
Days 7-13 neurohypophysis IFL
tlv05Tg ; zf44Tg ; c264Tg control Days 7-13 hypophysis IFL
mCherry tlv05Tg ; biu5Tg ; c264Tg control Days 7-13 adenohypophysis IFL
tlv05Tg ; c264Tg ; s843Tg control Days 7-13 adenohypophysis IFL
tlv05Tg ; wz01Tg ; c264Tg control Day 5 preoptic area IFL
Days 7-13 adenohypophysis IFL
tlv05Tg ; zf44Tg ; c264Tg control Days 7-13 adenohypophysis IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.