FIGURE

Fig. 10

ID
ZDB-FIG-171228-27
Publication
England et al., 2017 - Identification and Expression Analysis of the Complete Family of Zebrafish pkd Genes.
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Fig. 10

Expression of pkd genes in kidney, somites and sensory organs. (A–D) Lateral view of pkd1 (A,B) or pkd2 (C,D) expression in pronephros (black and white arrows) at 27 and 36 h. Rostral left, dorsal up. pkd1 is strongly expressed in pronephros at 27 h (A). Expression starts to decline at 36 h (B). Expression of pkd2 is weak in pronephros at 27 h (C) and is reduced even further by 36 h (D). (E–H) Lateral expression of pkd genes in the ear at 4–5 dpf. Dotted line shows ear boundary. Weak expression of pkd1l1 (E) and pkd2l1 (G) is first detected at 4 dpf in the inner ear ectoderm that supports the posterior canal and posterior crista (black arrowheads). pkd1l1 is also weakly expressed in the utricular otolith (white arrows). By 5 dpf, pkd1l1 expression persists in the utricular otolith and the underlying utricular macula (white arrows). It is also expressed in neighboring ectoderm flanking the lateral canal and lateral crista (white asterisks; F). At 5 dpf the expression of pkd2l1 persists in tissue surrounding the posterior canal and posterior crista (black arrowheads; H). (I–M) Lateral view of pkd1 expression in neuromasts (white asterisks) and lateral line primordium (white dotted line) at 36 h and 3 dpf. Rostral left, dorsal up. Weak expression of pkd1 in neuromasts and lateral line primordium is first detected at 36 h [I, higher magnification of the neuromasts (J) and lateral line primordium (K)]. By 3 dpf expression persists in neuromasts (L and higher magnification view, M). pkd1 is also expressed in pectoral fin buds (black arrows) at 36 h [dorsal view, rostral top (N), and lateral view—rostral left, dorsal up (O)]. (P–R) Lateral expression of pkd2 in rostral somites at 4 and 5 dpf. Rostral left, dorsal up. pkd2 is first expressed in the ventral half of each rostral somite at 4 dpf (black arrows in P, higher magnification in Q) and persists at 5 dpf (black arrows in R). (S–W) Lateral expression of pkd genes in the eye at 4 dpf. Rostral left, dorsal up. pkd1b, pkd1l1, pkd1l2a, pkd1l2b, and pkd2l1 are expressed in the ganglion cell layer (adjacent to lens, single white cross) and amacrine cells (outer cell layer immediately adjacent to ganglion cell layer, double white cross) of the eye at 4 dpf. The expression of pkd1b (S) and pkd1l2b is weak (V) and the expression of pkd1l1 (T), pkd1l2a (U), and pkd2l1 (W) is stronger. Only the expression of pkd1l2b persists in these cell layers at 5 dpf (data not shown). Scale bar (A) = 23 μm (J,K,M); 42 μm (E–H,O); 50 μm (A–D,Q,R); 55 μm (N); 62.5 μm (S–W); and 100 μm (I,L,P).

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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