FIGURE

Fig. 8

ID

ZDB-FIG-170901-9

Publication

Gau et al., 2017 - An ancient neurotrophin receptor code; a single Runx/Cbfβ complex determines somatosensory neuron fate specification in zebrafish

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Fig. 8

Loss of runx or cbfb expression affects ion channel expression in the RBs.

A-L, Colormetric in situ hybridization for trpv1 (A-D), trpa1b (E-H), and piezo2b (I-L) in the runx1^W84X/W84X, runx3^w144/w144 and cbfb^w128/w128 mutants focusing on the RBs at 24hpf and 3dpf. M-P, Antibody staining of GFP (green) in runx3^w144/w144; trpa1b:GFP and cbfb^w128/w128; trpa1b:GFP mutants. Q-R, Quantification of marker gene expression as total number of RB neurons/embryo and as % WT at 24hpf. S, Quantification of GFP+ cells in the RBs at 24hpf. Dashed line outlines the eye; Arrow, RBs; Arrowhead, TG; X, vagal ganglion. Scale bar: A-L 100 μm, J-L, M-N 20μm. Embryos per condition (n = 3–12). ***p<0.001. All error bars represent S.E.M.

Expression Data

Genes:	EGFP piezo2b trpa1b trpv1
Fish:	WT cbfb^w128/w128 (AB) runx1^hg1/hg1 runx3^w144/w144 (AB) a129Tg cbfb^w128/w128; a129Tg (AB) runx1^hg1/hg1; a129Tg runx3^w144/w144; a129Tg (AB)
Anatomical Term:	Rohon-Beard neuron
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cbfb^w128/w128 (AB) runx1^hg1/hg1 runx3^w144/w144 (AB) cbfb^w128/w128; a129Tg (AB) runx1^hg1/hg1; a129Tg runx3^w144/w144; a129Tg (AB)
Observed In:	Rohon-Beard neuron
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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