FIGURE

Fig. S6

ID

ZDB-FIG-170726-4

Publication

Ferre-Fernández et al., 2017 - Whole-Exome Sequencing of Congenital Glaucoma Patients Reveals Hypermorphic Variants in GPATCH3, a New Gene Involved in Ocular and Craniofacial Development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S6

Zebrafish gpatch3 morphants present similar gross morphological alterations with three different morpholinos.

Control, Gpatch3^ATG, Gpatch3^ex1/2 and Gpatch3^ex2/3 MOs (2.0 ng) were microinjected in 1-2 cells zebrafish embryos. The standard negative control consisted of a MO that targets a human beta-globin intron mutation that causes beta-thalassemia. At 72 hours post fecundation (hpf), larvae were analyzed. Dorsal, lateral and ventral dark field micrographs show altered phenotypes including microphthalmia (blue arrowheads) and abnormal development of pectoral fins (green arrowheads) and craniofacial structures including absence of jaw cartilages (red arrowheads). Scale bars indicate 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-gpatch3 TU + MO2-gpatch3 TU + MO3-gpatch3
Knockdown Reagents:	MO1-gpatch3 MO2-gpatch3 MO3-gpatch3
Observed In:	eye mandibular arch skeleton pectoral fin
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.