Fig. 5

Thomas-Jinu et al., 2017 - Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

SFPQ Protein Is Found in Axons, with Unprocessed RNA Targets and Spliceosome Component U1 snRNP70

Transverse single confocal sections of 24 hpf (A, N, and O) and 48 hpf (B–M) forebrain (A and I–K) or spinal cord dorsal to the top (G, H, L, and M) or to the left (B–D), and lateral view of head, anterior to the left (E and F).

(A–F) SFPQ protein expression shown by antibody staining (A–D) or GFP-tagged ectopically expressed protein (E and F). Identity of red, green, and blue staining is labeled on bottom right corner of each picture. Arrow shows axonal localization.

(G–K) Localization of first alternative introns (in red) of SFPQ-dependent transcripts, bcas3 (G and H), and gnao1a (I–K). Axons are stained by acetylated tubulin antibody in green.

(L and M) High-resolution imaging of bcas3 intron detection (L; red) in motor axons stained with acetylated tubulin antibody (M; green).

(N and O) Double antibody staining of U1 snRNP70 proteins (N) with acetylated tubulin (O; merged channels) in spinal axons. Arrows are pointing to motor axons. Scale bar, 25 μm.

Expression Data
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron