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ZIRC
ZFIN ID: ZDB-FIG-170516-49
Sauteur et al., 2017 - Distinct and redundant functions of Esam and VE-cadherin during vascular morphogenesis. Development (Cambridge, England)   144(8):1554-1565 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to High-pec

Fig. 3

Loss of both VE-cad and Esama enhances the defects observed in ve-cadubs8 mutants. (A-D) Still images from Movie 4 (A), Movie 5 (B), Movie 6 (C) and Movie 7 (D) of Tg(fli1a:EGFP)y1 wild-type, esamaubs19, ve-cadubs8 and esamaubs19; ve-cadubs8 double mutant (dKO) embryos, respectively. Images are shown in inversed contrast at three different stages of angiogenesis: ∼32, ∼38 and ∼44 hpf. Red arrowheads at 32 hpf point to anastomotic contacts; red arrowheads at 38 hpf point to forming lumen; green arrowheads point to stalk cells that detached from the tip cell. Scale bars: 20 µm. (E and F) Quantification of filopodial protrusions on SeA stalks (E) and tip cells (F). (E) The number of filopodia on angiogenic stalks (composed of one to three ECs) is comparable between wild-type (77 SeAs in n=20 embryos), esamaubs19 (114 SeAs in n=30 embryos) and ve-cadubs8 (31 SeAs in n=9 embryos) genotypes. Significantly more filopodia were counted on dKO stalks (31 SeAs in n=8 embryos). (F) Tip cells generally generate more filopodial protrusions (average of 50 filopodia per tip cell) than stalk cells. Significantly more filopodia were counted on tip cells of dKO compared with wild type. Bars in the plots represent mean±s.d. DA, dorsal aorta; dKO, double mutant; DLAV, dorsal longitudinal anastomotic vessel; n, number of analyzed embryos; ns, not significant; SA, segmental artery. ***P<0.0001, with one-way analysis of variance (ANOVA).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ubs18Tg ; s916Tg ; ubs3Tg ; cdh5ubs8/ubs8 standard conditions Prim-15 angiogenic sprout has normal numbers of parts of type endothelial cell filopodium, normal
Prim-15 endothelial tip cell cell adhesion decreased efficacy, abnormal
Prim-25 angiogenic sprout endothelial tip cell detached from angiogenic sprout, abnormal
Prim-25 endothelial tip cell cell adhesion decreased efficacy, abnormal
High-pec endothelial tip cell cell adhesion decreased efficacy, abnormal
ubs18Tg ; ubs3Tg ; s916Tg ; esamaubs19/ubs19 standard conditions Prim-15 angiogenic sprout has normal numbers of parts of type endothelial cell filopodium, normal
Prim-15 intersegmental vessel morphology, normal
Prim-25 dorsal longitudinal anastomotic vessel morphology, normal
Prim-25 intersegmental vessel morphology, normal
High-pec dorsal longitudinal anastomotic vessel morphology, normal
High-pec intersegmental vessel morphology, normal
ubs3Tg ; cdh5ubs8/ubs8 ; s916Tg ; esamaubs19/ubs19 ; ubs18Tg standard conditions Prim-15 angiogenic sprout has extra parts of type endothelial cell filopodium, abnormal
Prim-15 dorsal longitudinal anastomotic vessel blood vessel development delayed, abnormal
Prim-15 endothelial tip cell actin filament bundle of filopodium decreased amount, abnormal
Prim-15 endothelial tip cell cell adhesion decreased efficacy, exacerbated
Prim-25 angiogenic sprout endothelial tip cell detached from angiogenic sprout, exacerbated
Prim-25 dorsal longitudinal anastomotic vessel blood vessel development delayed, abnormal
Prim-25 endothelial tip cell actin filament bundle of filopodium decreased amount, abnormal
Prim-25 endothelial tip cell cell adhesion decreased efficacy, exacerbated
High-pec angiogenic sprout endothelial tip cell detached from angiogenic sprout, exacerbated
High-pec dorsal longitudinal anastomotic vessel blood vessel development delayed, abnormal
High-pec endothelial tip cell aggregated, abnormal
High-pec endothelial tip cell actin filament bundle of filopodium decreased amount, abnormal
High-pec endothelial tip cell cell adhesion decreased efficacy, exacerbated
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development