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ZIRC
ZFIN ID: ZDB-FIG-170516-48
Sauteur et al., 2017 - Distinct and redundant functions of Esam and VE-cadherin during vascular morphogenesis. Development (Cambridge, England)   144(8):1554-1565 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 2

In the absence of VE-cad, several filopodial contacts are required to initiate cell-cell contact formation. (A-F) Still images from Movie 2 of a VE-cad morphant Tg(fli1ep:gal4ff)ubs3, Tg(UAS:EGFP-UCHD)ubs18, Tg(kdrl:mCherry-CAAX)s916 embryo at around 32 hpf, with anterior to the left. Single channels are shown in inversed contrast (green is EGFP-UCHD and red is mCherry-CAAX on left and right, respectively) and the merge is shown in the middle. Red arrowheads point to filopodial interactions or cell bodies touching in (E); green arrowheads point to cell-cell bridges and forming junctions in (E) and (F). Scale bar: 10 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ubs18Tg ; s916Tg ; ubs3Tg + MO1-cdh5 standard conditions Prim-15 intersegmental vessel actin filament bundle of filopodium IFL
mCherry ubs18Tg ; s916Tg ; ubs3Tg + MO1-cdh5 standard conditions Prim-15 intersegmental vessel filopodium membrane IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ubs18Tg ; s916Tg ; ubs3Tg + MO1-cdh5 standard conditions Prim-15 cell adhesion involved in sprouting angiogenesis disrupted, abnormal
ubs18Tg ; s916Tg ; ubs3Tg + MO1-cdh5 standard conditions Prim-15 dorsal longitudinal anastomotic vessel blood vessel development delayed, abnormal
ubs18Tg ; s916Tg ; ubs3Tg + MO1-cdh5 standard conditions Prim-15 endothelial tip cell cell adhesion decreased efficacy, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development