ZFIN ID: ZDB-FIG-170306-3
Gays et al., 2017 - An exclusive cellular and molecular network governs intestinal smooth muscle cell differentiation in vertebrates. Development (Cambridge, England)   144(3):464-478 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 2

drl-positive LPM cells give rise to iSMC. (A) Schematic of the drl:creERT2×ubi:lox-EGFP-lox-mCherry (ubi:switch) crosses. Double-positive embryos were induced at the one-somite stage with 4-OH tamoxifen (10 µM final concentration). This activates the Cre recombinase, which then excises the loxP-flanked EGFP cassette and brings mCherry under control of the ubi promoter to lineage trace the switched cells. Photomicrographs of transverse vibratome sections of posterior trunk region (dr:creERT2;ubi:Switch) are shown below. Sections were imaged with a Zeiss LSM710 40× objective. Scale bar: 50 µm. Higher magnification of the intestinal region. The merged channel comprises EGFP, mCherry and DAPI. (B) Transverse vibratome sections of the posterior trunk region (dr:creERT2;ubi:Switch). Higher magnifications of the intestinal region. iSMCs are stained using transgelin antibody to compare with lineage labeling by drl:creERT2. Scale bar: 30 µm. The merged channel comprises EGFP, mCherry and DAPI. (C) Transverse vibratome sections of the posterior trunk region (drl:creERT2xubi:switch). Higher magnification of the intestinal region showing the different switching efficacy for iSMCs after 4-OH treatment at the one-somite stage. Class I, few iSMC are switched; class II, half iSMC are switched; class III, the entire population of iSMCs surrounding the gut are switched. The occurrences of the switching efficacies are: class I, 28% (9/31); class II, 50% (15/31); class III, 22% (7/31). Asterisks indicate switched iSMCs. Sections were imaged with a Zeiss LSM710 40× objective. Scale bar: 25 µm. The merged channel comprises EGFP, mCherry and DAPI.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP cz1701Tg ; cz3333Tg control Protruding-mouth whole organism IHC
cz1701Tg ; cz3333Tg chemical treatment: afimoxifene Protruding-mouth whole organism IHC
mCherry cz1701Tg ; cz3333Tg control Protruding-mouth Not Detected anatomical structure IHC
Protruding-mouth Not Detected gut anatomical region IHC
cz1701Tg ; cz3333Tg chemical treatment: afimoxifene Protruding-mouth intestine smooth muscle IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tagln IHC cz1701Tg ; cz3333Tg chemical treatment: afimoxifene Protruding-mouth intestine smooth muscle
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development