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ZFIN ID: ZDB-FIG-170306-2
Gays et al., 2017 - An exclusive cellular and molecular network governs intestinal smooth muscle cell differentiation in vertebrates. Development (Cambridge, England)   144(3):464-478 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 1

LPM gives rise to iSMCs in zebrafish embryos. (A) Time-course analyses of Tg(hand2:EGFP)pd24 and iSMC marker expression (Tagln) during intestinal development. Tg(hand2:EGFP)pd24 embryos were fixed at different time points from 24 hpf until 96 hpf. Upper panel: confocal transverse sections of the posterior gut region between the somites 7 and 13 of Tg(hand2:EGFP)pd24 embryos stained with phalloidin (gray) and Tagln (red) (single channels are shown in Fig. S1A). The dashed yellow lines highlight LPM/hand2+ cells, whereas the dashed white lines highlight the enteric endoderm (g). Migration of the LPM is indicated by arrows. Asterisks indicate single-cell nuclei. ISMC differentiation is visible during intestinal development by expression of Tagln; blue indicates nuclei; g, gut. Scale bar: 30 μm. Bottom panel: schematic representation of LPM/hand2+ conversion to iSMCs in the gut region of developing zebrafish embryos. Green, LPM; pink, endoderm; red, iSMCs; p, pronephros; s, somite; PCV, posterior cardinal vein; y, yolk. (B) Analyses of Tg(hand2:EGFP)pd24 and polarity and mesenchymal markers during LPM development at 24 hpf. Confocal transverse sections of the posterior gut region between the somites 7 and 13 of Tg(hand2:EGFP)pd24 embryos stained with aPKC or N-cadherin. Nuclei are in blue; g, gut. Scale bars: 30 μm. Asterisks indicate single-cell nuclei while the dashed yellow lines highlight LPM/hand2+ cells. (C) Analyses of Tg(hand2:EGFP)pd24 and polarity and mesenchymal markers during LPM development at 48 hpf. Confocal transverse sections of the posterior gut region between the somites 7 and 13 of Tg(hand2:EGFP)pd24 embryos stained with aPKC (left, red) or N-cadherin (right, red). Blue indicates nuclei; g, gut. Scale bars: 30 μm. (D) Analyses of Tg(hand2:EGFP)pd24 and iSMC marker expression (Tagln) at 72 hpf. Confocal transverse sections of the posterior gut region between the somites 7 and 13 of Tg(hand2:EGFP)pd24 embryos stained with Tagln (red) show that all differentiated iSMC are also Tg(hand2:EGFP)pd24 positive. These observations suggest that posterior LPM expression of hand2 does not demarcate the entire LPM, but rather is confined to the presumptive iSMC progenitors from its expression onset after LPM formation. Nuclei are in blue; g, gut. Scale bars: 30 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pd24Tg control Prim-5 epithelial cell IHC
Prim-5 lateral plate mesoderm IHC
Prim-5 mesenchymal cell IHC
Prim-25 gut anatomical region IHC
Prim-25 mesoderm IHC
Long-pec epithelial cell IHC
Long-pec gut anatomical region IHC
Long-pec mesenchymal cell IHC
Long-pec mesoderm IHC
Protruding-mouth gut anatomical region IHC
Protruding-mouth intestine smooth muscle IHC
Day 4 gut anatomical region IHC
Day 4 intestine smooth muscle IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-tagln IHC pd24Tg control Prim-5 Not Detected gut anatomical region
IHC Prim-25 Not Detected gut anatomical region
IHC Long-pec Not Detected gut anatomical region
IHC Protruding-mouth gut anatomical region
IHC Protruding-mouth intestine smooth muscle
IHC Day 4 gut anatomical region
IHC Day 4 intestine smooth muscle
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development