ZFIN ID: ZDB-FIG-170104-13
Jiménez-Amilburu et al., 2016 - In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation. Cell Reports   17:2687-2699 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 6

Nrg2a Signaling Is Required for Notch Reporter Activity in Zebrafish CMs

(A–D'') Confocal images (mid-sagittal sections) of 96-hpf Tg(TP1:VenusPEST) zebrafish larvae from a nrg2a+/− incross (A–B''). Images show a number of Notch-reporter-positive CMs in the compact layer in the presence of Nrg2a function (A–A'', arrows), while the number of TP1:VenusPEST-positive CMs is dramatically reduced in the absence of Nrg2a function (B–B'', Ø). (C) Graph showing the number of TP1:VenusPEST-positive CMs per 96-hpf heart in nrg2a+/− (n = 11) versus nrg2a−/− (n = 8) larvae. Each dot represents one heart. Data are shown as mean ± SEM. ∗∗∗p < 0.001 by Student’s t test.

(D–F) Confocal images (mid-sagittal section) of 96-hpf Tg(TP1:VenusPEST) larval heart. Non-injected larvae show TP1:VenusPEST-positive CMs in the compact layer (D, arrows). tnnt2a morphants show absence of Tg(TP1:VenusPEST) expression in CMs (E, Ø). In tnnt2a morphants, mosaic expression of nrg2a in CMs activates Tg(TP1:VenusPEST) expression in neighboring CMs (F, arrows).

(G) Graph shows the percentage of hearts showing TP1:VenusPEST positive CMs at 96 hpf in non-injected larvae (n = 18), tnnt2a morphants (n = 31) and tnnt2a morphants overexpressing nrg2a in the myocardium (n = 23).

Yellow dashed line outlines the tnnt2a morphant heart (E). At, atrium; V, ventricle; AVC, atrioventricular canal. Scale bars, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
Venus nrg2amn0237Gt/mn0237Gt ; s940Tg control Day 4 atrioventricular valve IFL
Day 4 cardiac muscle cell IFL
s940Tg control Day 4 atrioventricular valve IFL
Day 4 cardiac muscle cell IFL
s940Tg + MO1-tnnt2a control Day 4 Not Detected atrioventricular valve IFL
Day 4 Not Detected cardiac muscle cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nrg2amn0237Gt/mn0237Gt ; s940Tg control Day 4 cardiac muscle cell Venus expression decreased amount, abnormal
nrg2amn0237Gt/mn0237Gt ; s940Tg control Day 4 cardiac muscle cell Notch signaling pathway decreased process quality, abnormal
s940Tg + MO1-tnnt2a control Day 4 atrioventricular valve Venus expression absent, abnormal
Day 4 cardiac muscle cell Venus expression absent, abnormal
Day 4 cardiac muscle cell Notch signaling pathway decreased process quality, abnormal
Day 4 heart Notch signaling pathway decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.