ZFIN ID: ZDB-FIG-170104-14
Jiménez-Amilburu et al., 2016 - In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation. Cell Reports   17:2687-2699 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 7

Blocking Notch Activation Did Not Increase the Number of CMs Exhibiting Apical Constriction or of CMs in the Trabecular Layer

(A–B') Confocal images (mid-sagittal sections) of 81-hpf Tg(myl7:MKATE-CAAX);Tg(TP1:VenusPEST) zebrafish hearts. DMSO-treated larvae show Notch activity in CMs (A and A', asterisks) and atrioventricular canal (AVC) cells (A and A', yellow arrows). Larvae treated with 100 μM DAPT lack Notch activity in both CMs and AVC cells (B and B', Ø).

(C–D') Confocal images (mid-sagittal sections) of 79-hpf Tg(−0.2myl7:EGFP-podxl);Tg(myl7:MKATE-CAAX);Tg(TP1:H2B-mCherry) zebrafish hearts. DMSO-treated larvae show CM apical constrictions (C and C', arrowheads). 79-hpf larvae treated at 48 hpf with 100 μM DAPT show similar numbers of CM apical constrictions than controls (D and D', arrowheads).

(E and F) Number of CM apical constrictions per ventricle at the mid-sagittal plane after 100 μM DAPT treatment. Animals were treated at 48 hpf and imaged at 79 hpf (E) or treated at 60 hpf and imaged at 72 hpf (F). Each dot represents one heart. Data are shown as mean ± SEM. ns, no significant differences by Student’s t test.

(G–H') Confocal images (mid-sagittal sections) of 79-hpf Tg(−0.2myl7:EGFP-podxl);Tg(myl7:MKATE-CAAX);Tg(myl7:nDsRed2) zebrafish hearts. DMSO-treated larvae show normal CM shape in the compact layer (G', arrowheads), while DAPT-treated larvae show abnormal CM shape (H', arrowheads).

(I and J) Graphs showing the percentage of trabecular CMs versus the total number of CMs counted in two regions of the outer curvature. Each dot represents one heart.

Data are shown as mean ± SEM. p < 0.05 and ∗∗∗p < 0.001 by Student’s t test. V, ventricle; AVC, atrioventricular canal. Scale bars, 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed2 f2Tg ; sd11Tg ; bns103Tg control Protruding-mouth cardiac muscle cell nucleus IFL
f2Tg ; sd11Tg ; bns103Tg chemical treatment: DAPT Protruding-mouth cardiac muscle cell nucleus IFL
EGFP f2Tg ; sd11Tg ; bns103Tg control Protruding-mouth cardiac muscle cell apical plasma membrane IFL
f2Tg ; sd11Tg ; bns103Tg chemical treatment: DAPT Protruding-mouth cardiac muscle cell apical plasma membrane IFL
s939Tg ; bns103Tg ; sd11Tg control Protruding-mouth cardiac muscle cell apical plasma membrane IFL
s939Tg ; bns103Tg ; sd11Tg chemical treatment: DAPT Protruding-mouth cardiac muscle cell apical plasma membrane IFL
mCherry s939Tg ; bns103Tg ; sd11Tg control Protruding-mouth atrioventricular valve IFL
Protruding-mouth cardiac muscle cell IFL
s939Tg ; bns103Tg ; sd11Tg chemical treatment: DAPT Protruding-mouth atrioventricular valve IFL
Protruding-mouth cardiac muscle cell IFL
MKATE2 f2Tg ; sd11Tg ; bns103Tg control Protruding-mouth cardiac muscle cell membrane IFL
f2Tg ; sd11Tg ; bns103Tg chemical treatment: DAPT Protruding-mouth cardiac muscle cell membrane IFL
s939Tg ; bns103Tg ; sd11Tg control Protruding-mouth cardiac muscle cell membrane IFL
s939Tg ; bns103Tg ; sd11Tg chemical treatment: DAPT Protruding-mouth cardiac muscle cell membrane IFL
s940Tg ; sd11Tg control Protruding-mouth cardiac muscle cell membrane IFL
s940Tg ; sd11Tg chemical treatment: DAPT Protruding-mouth cardiac muscle cell membrane IFL
Venus s940Tg ; sd11Tg control Protruding-mouth atrioventricular valve IFL
Protruding-mouth cardiac muscle cell IFL
s940Tg ; sd11Tg chemical treatment: DAPT Protruding-mouth Not Detected atrioventricular valve IFL
Protruding-mouth Not Detected cardiac muscle cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
f2Tg ; sd11Tg ; bns103Tg chemical treatment: DAPT Protruding-mouth cardiac muscle cell shape, abnormal
f2Tg ; sd11Tg ; bns103Tg chemical treatment: DAPT Protruding-mouth trabecular layer cardiac muscle cell decreased amount, abnormal
s940Tg ; sd11Tg chemical treatment: DAPT Protruding-mouth atrioventricular valve Venus expression absent, abnormal
Protruding-mouth cardiac muscle cell Venus expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.