ZFIN ID: ZDB-FIG-170104-12
Jiménez-Amilburu et al., 2016 - In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation. Cell Reports   17:2687-2699 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Days 7-13

Fig. 5

Notch-Signaling-Reporter-Positive CMs Appear in the Compact Layer Adjacent to CMs Undergoing Apical Constriction

(A–C) Confocal images (mid-sagittal sections) of Tg(myl7:ras-mCherry);Tg(TP1:VenusPEST) zebrafish larvae at 76 (A), 100 (B), and 125 (C) hpf. Asterisks indicate CMs expressing the Notch signaling reporter. White arrows in (A) point to fading TP1:VenusPEST expression in the endocardium. Yellow arrows point to the developing AV valve in (A) and (B).

(D–F) Confocal sections of 7-dpf larval hearts obtained from crossing the Tg(TP1:CreERT2) line to three different indicator lines. In each case, embryos were treated with 4-OHT from 2 to 5 dpf. Asterisks indicate labeled CMs in the compact layer.

(D) Lineage-traced 7-dpf Tg(TP1:CreERT2);Tg(myl7:RSG) heart. Arrowhead points to a CM neighboring the AV canal.

(E) Lineage-traced 7 dpf Tg(TP1:CreERT2);Tg(β-act2:RSG) heart.

(F) Lineage-traced 7 dpf Tg(TP1:CreERT2);Tg(ubi:Switch) heart. Note that in this case, lineage-traced cells are red. White arrows point to labeled endocardial cells.

(G–J') Confocal images (mid-sagittal sections) of 79-hpf Tg(−0.2myl7:EGFP-podxl);Tg(myl7:MKATE-CAAX);Tg(TP1:H2B-mCherry) zebrafish hearts. Boxed areas in (G) and (H) are shown in G' and H', respectively. Notch-reporter-positive CMs (G' and H', asterisks) localize in the compact layer adjacent to CMs undergoing apical constriction (G' and H', white arrowheads). Boxed areas in (I) and (J) are shown in (I') and (J'), respectively. Notch-reporter-positive CMs (I' and J', asterisks) appear directly adjacent to a delaminated CM (I' and J', //). Purple arrowheads point to Notch-reporter-positive endocardial cells (G', H', I', and J').

(K) Graph shows percentage of CMs exhibiting apical constriction that are surrounded by Notch-reporter-positive CMs per heart at 79 hpf. Each dot represents one heart.

At, atrium; V, ventricle; AVC, atrioventricular canal. Scale bars, 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed s959Tg ; s928Tg chemical treatment by environment: tamoxifen Days 7-13 cardiac muscle cell IFL
EGFP cz1701Tg; s959Tg chemical treatment by environment: tamoxifen Days 7-13 cardiac muscle cell IFL
s939Tg ; bns103Tg ; sd11Tg control Protruding-mouth cardiac muscle cell apical plasma membrane IFL
s959Tg ; s738Tg chemical treatment by environment: tamoxifen Days 7-13 cardiac muscle cell IFL
s959Tg ; s928Tg chemical treatment by environment: tamoxifen Days 7-13 cardiac muscle cell IFL
mCherry cz1701Tg; s959Tg chemical treatment by environment: tamoxifen Days 7-13 cardiac muscle cell IFL
Days 7-13 endocardium IFL
s939Tg ; bns103Tg ; sd11Tg control Protruding-mouth atrioventricular valve IFL
Protruding-mouth cardiac muscle cell IFL
Protruding-mouth endocardium IFL
s940Tg ; sd21Tg control Protruding-mouth cardiac muscle cell IFL
Day 4 cardiac muscle cell IFL
Day 5 cardiac muscle cell IFL
s959Tg ; s738Tg chemical treatment by environment: tamoxifen Days 7-13 cardiac muscle cell IFL
MKATE2 s939Tg ; bns103Tg ; sd11Tg control Protruding-mouth cardiac muscle cell membrane IFL
Venus s940Tg ; sd21Tg control Protruding-mouth atrioventricular valve IFL
Protruding-mouth cardiac muscle cell IFL
Protruding-mouth endocardium IFL
Day 4 atrioventricular valve IFL
Day 4 cardiac muscle cell IFL
Day 5 cardiac muscle cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.