ZFIN ID: ZDB-FIG-161222-37
Alvarado et al., 2016 - Wdr68 Mediates Dorsal and Ventral Patterning Events for Craniofacial Development. PLoS One   11:e0166984 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Prim-5

Fig. 8

dlx1a and dlx2a expression is wdr68-dependent and responsive to inhibition of TGF-β signaling in wdr68hi3812/hi3812 zebrafish.

(A-H) ISH analysis of prim-12 stage embryos raised at 32°C. A-F) dlx2a expression with red underline for anterior portion of 1st arch and blue underline for 2nd arch. A, C, E) lateral view. B, D, F) dorso-lateral view. A, B) DMSO-treated wildtype sibling showing normal dlx2a. C, D) DMSO-treated wdr68hi3812/hi3812 mutant showing loss of anterior 1st arch dlx2a. E, F) SB431542-treated wdr68hi3812/hi3812 mutant showing partial rescue of anterior 1st arch dlx2a. G, H) dlx1a expression. G) wildtype sibling showing normal dlx1a. H) wdr68hi3812/hi3812 mutant showing loss of anterior 1st arch dlx1a.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx1a WT heat exposure Prim-5 pharyngeal arch 1 ISH
dcaf7hi3812Tg/hi3812Tg heat exposure Prim-5 pharyngeal arch 1 anterior region ISH
dlx2a WT heat exposure Prim-5 pharyngeal arch 1 cranial neural crest ISH
Prim-5 pharyngeal arch 2 cranial neural crest ISH
dcaf7hi3812Tg/hi3812Tg heat exposure Prim-5 pharyngeal arch 1 anterior region ISH
Prim-5 pharyngeal arch 2 cranial neural crest ISH
dcaf7hi3812Tg/hi3812Tg heat exposure, chemical treatment by environment: SB 431542 Prim-5 pharyngeal arch 1 ISH
Prim-5 pharyngeal arch 2 cranial neural crest ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dcaf7hi3812Tg/hi3812Tg heat exposure Prim-5 pharyngeal arch 1 anterior region dlx1a expression decreased distribution, abnormal
Prim-5 pharyngeal arch 1 anterior region dlx2a expression decreased distribution, abnormal
dcaf7hi3812Tg/hi3812Tg heat exposure, chemical treatment by environment: SB 431542 Prim-5 pharyngeal arch 1 dlx2a expression spatial pattern, ameliorated
Acknowledgments:
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