FIGURE

Fig. 4

ID

ZDB-FIG-161222-11

Publication

Eum et al., 2016 - 3D Visualization of Developmental Toxicity of 2,4,6-Trinitrotoluene in Zebrafish Embryogenesis Using Light-Sheet Microscopy

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Fig. 4

3D light-sheet/SPIM imaging of the TNT circulation defect. The vasculature (green) and blood cells (red) of the posterior trunk were visualized in Tg(gata1:DsRed/fli1a:EGFP) embryos at 38 hpf. 3D reconstructions are shown at lateral view (a,c) and transverse view (b,d), using Arivis software (surface function). (a,b) Embryos treated for 33 h with control E3 media; (c,d) Embryos treated for 33 h with pink water containing 13.5 µg/mL TNT showing swollen body structure with abnormal blood islands (arrowhead) and blood accumulation (arrow). 20X water lens, 0.8 zoom. Scale bar, 150 µm. BI, blood Island; CA, caudal artery; CV, caudal vein; DA, dorsal aorta; ISV, intersegmental vessel. The 3D movies of the reconstructions are presented in Supplementary video 2A,B.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.