ZFIN ID: ZDB-FIG-160317-38
Liu et al., 2016 - Cannabinoid receptor signaling regulates liver development and metabolism. Development (Cambridge, England)   143:609-22 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. S1

Cannabinoid receptor expression in the developing zebrafish

A) FACS quantification of GFP-positive hepatocytes in fabp10a:GFP embryos treated with cannabinoid agonists from 24-72hpf. % of GFP positive cells were normalized to controls. MeanĀ±s.e.m., n=5 groups of 10 pooled embryos; p=0.08, one-way ANOVA analysis.

B) Dorsal and lateral in situ hybridization images at 24, 48, and 72hpf showing that cnr1 and cnr2 are expressed in the developing central nervous system, endoderm (arrow), and liver regions (arrow). Scale bar = 0.2 mm.

C) qRT-PCR detects cnr1 expression in pooled FACS-sorted GFP-positive cells (~200,000 cells) from wild type fabp10a:GFP normalized to expression in the GFP-negative cell fraction, which includes the central nervous system. cnr2 is detectable in the non-hepatocyte (GFP-negative) fraction, but not in the hepatocytes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cnr1 WT standard conditions Prim-5 central nervous system ISH
Long-pec central nervous system ISH
Protruding-mouth central nervous system ISH
Protruding-mouth liver ISH
Day 4 liver ISH
as3Tg standard conditions Protruding-mouth hepatocyte RTPCR
cnr2 WT standard conditions Prim-5 central nervous system ISH
Long-pec central nervous system ISH
Protruding-mouth central nervous system ISH
Protruding-mouth liver anatomical region ISH
Day 4 liver anatomical region ISH
as3Tg standard conditions Protruding-mouth Not Detected hepatocyte RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
as3Tg chemical treatment: cannabinoid receptor agonist Protruding-mouth hepatocyte increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development