ZFIN ID: ZDB-FIG-160311-58
Stapel et al., 2016 - Automated detection and quantification of single RNAs at cellular resolution in zebrafish embryos. Development (Cambridge, England)   143(3):540-6 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Sphere

Fig. 4

smFISH provides quantitative subcellular information on gene expression. Detected transcripts (magenta) and transcription foci (white) for eif4g2a (A), tbx16 (B) and akap12b (C) at sphere stage. Green, cell outlines; blue, nuclear outlines. Scale bars: 10µm. Images are maximum projections of 17 z-slices spaced by 0.3µm. (D) Single transcript quantification. Values are averages from sections of three different embryos. Error bars represent s.e.m. (E) smFISH for akap12b (left) with corresponding membrane staining for Phalloidin (right) at dome stage. Images are single z-slices. Scale bars: 10 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
akap12b AB/TL standard conditions Sphere whole organism cytoplasm ISH
Sphere whole organism nucleus ISH
Dome whole organism plasma membrane ISH
eif4g2a AB/TL standard conditions Sphere whole organism cytoplasm ISH
Sphere whole organism nucleus ISH
tbx16 AB/TL standard conditions Sphere whole organism cytoplasm ISH
Sphere whole organism nucleus ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development