ZFIN ID: ZDB-FIG-160215-15
Edmunds et al., 2016 - Phenoscape: Identifying candidate genes for evolutionary phenotypes. Molecular Biology and Evolution   33(1):13-24 Full text @ Mol Bio Evol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: brpf1
Fish: WT
Anatomical Terms: ceratobranchial 1 cartilage, ceratobranchial 2 cartilage, ceratobranchial 3 cartilage, ceratobranchial 4 cartilage, ceratohyal cartilage
Stage: Protruding-mouth

Fig. 5

Catfishes lack brpf1 expression in the region where the basihyal forms in zebrafish. Expression of brpf1 during pharyngeal arch skeletal development in Danio rerio and Ictalurus punctatus, as detected by whole-mount in situ hybridization. Staining of pharyngeal arch cartilages using (A) Alcian Blue in D. rerio (72 hpf) and (B) Alcian Green in I. punctatus (86 hpf). Endogenous brpf1 expression in (C) D. rerio (72 hpf) and (D) I. punctatus (86 hpf). Arrows indicate location of basihyal element in D. rerio (cypriniform) and lack of basihyal element in I. punctatus (siluriform). Images are representative of e30 embryos. No labeling was detected in negative controls (data not shown). m, Meckel’s cartilage; ch, ceratohyal cartilage; cb, ceratobranchial cartilages. Scale bars = 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
brpf1 WT standard conditions Protruding-mouth ceratobranchial 1 cartilage ISH
Protruding-mouth ceratobranchial 2 cartilage ISH
Protruding-mouth ceratobranchial 3 cartilage ISH
Protruding-mouth ceratobranchial 4 cartilage ISH
Protruding-mouth ceratohyal cartilage ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Molecular Biology and Evolution for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol Bio Evol