ZFIN ID: ZDB-FIG-160209-11
Hultin et al., 2014 - AmotL2 links VE-cadherin to contractile actin fibres necessary for aortic lumen expansion. Nature communications   5:3743 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 3 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkuasrfp1aTg; ubs12Tg; ubs3Tg standard conditions Prim-5 blood vessel endothelial cell cell-cell junction IFL
Prim-5 dorsal aorta blood vessel endothelial cell IFL
nkuasrfp1aTg; ubs12Tg; ubs3Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Prim-5 blood vessel endothelial cell cell-cell junction IFL
Prim-5 dorsal aorta blood vessel endothelial cell IFL
y1Tg standard conditions Prim-5 dorsal aorta blood vessel endothelial cell IFL
Prim-15 dorsal aorta blood vessel endothelial cell IFL
y1Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Prim-5 dorsal aorta blood vessel endothelial cell IFL
Prim-15 dorsal aorta blood vessel endothelial cell IFL
GFP mu101Tg; mu271Tg standard conditions Long-pec blood vessel endothelial cell filamentous actin ISH
Long-pec dorsal aorta blood vessel endothelial cell ISH
mu101Tg; mu271Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Long-pec blood vessel endothelial cell filamentous actin ISH
Long-pec dorsal aorta blood vessel endothelial cell ISH
RFP nkuasrfp1aTg; ubs12Tg; ubs3Tg standard conditions Prim-5 dorsal aorta blood vessel endothelial cell IFL
nkuasrfp1aTg; ubs12Tg; ubs3Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Prim-5 dorsal aorta blood vessel endothelial cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-lama1 IHC y1Tg standard conditions Prim-5 blood vessel endothelial cell basement membrane
IHC Prim-5 dorsal aorta blood vessel endothelial cell
IHC y1Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Prim-5 blood vessel endothelial cell basement membrane
IHC Prim-5 dorsal aorta blood vessel endothelial cell
Ab1-podxl2 IHC y1Tg standard conditions Prim-5 blood vessel endothelial cell apical plasma membrane
IHC Prim-5 dorsal aorta blood vessel endothelial cell
IHC y1Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Prim-5 blood vessel endothelial cell apical plasma membrane
IHC Prim-5 dorsal aorta blood vessel endothelial cell
Phenotype Details
Fish Conditions Stage Phenotype
mu101Tg; mu271Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Long-pec blood vessel endothelial cell actin filament disorganized, abnormal
Long-pec blood vessel endothelial cell filamentous actin punctate, abnormal
Long-pec dorsal aorta blood vessel endothelial cell malformed, abnormal
Long-pec dorsal aorta morphogenesis decreased process quality, abnormal
nkuasrfp1aTg; ubs12Tg; ubs3Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Prim-5 blood vessel endothelial cell cell-cell junction morphology, normal
y1Tg + MO1-amotl2a + MO1-amotl2b + MO2-amotl2b + MO3-amotl2b standard conditions Prim-5 blood vessel endothelial cell establishment of apical/basal cell polarity process quality, normal
Prim-15 dorsal aorta blood vessel endothelial cell decreased length, abnormal
Prim-15 dorsal aorta endothelial cell morphogenesis decreased process quality, abnormal
Prim-15 dorsal aorta morphogenesis decreased process quality, abnormal
Full text @ Nat. Commun.