Fig. 6
- ID
- ZDB-FIG-151229-19
- Publication
- Ariotti et al., 2015 - Modular Detection of GFP-Labeled Proteins for Rapid Screening by Electron Microscopy in Cells and Organisms
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APEX-GBP Can Detect Proteins Expressed at Endogenous Levels In Vivo (A) Schematic of GFP-CAAX zebrafish cross with Bact2-APEX-GBP zebrafish. (B) Confocal image of GFP-CAAX expressing zebrafish trunk muscle demonstrating GFP-CAAX is targeted to the sarcoplasmic reticulum. Green, GFP-CAAX; red, nuclei. (C) The APEX-GBP single transgenic zebrafish fish demonstrates soluble reaction product. (D) Electron micrograph of GFP-CAAX APEX-GBP double transgenic zebrafish muscle showing specific electron density at the PM of cells. Arrows highlight the electron density generated by the osmiophilic DAB reaction product accumulating at the site of the APEX-GBP modular tag and the GFP-CAAX protein in the double transgenic zebrafish line. Scale bars represent 1 µm. (E) Schematic of Dystrophin-YFP zebrafish cross with Bact2-APEX-GBP zebrafish. (F) Confocal image of Dystrophin-YFP expressing zebrafish trunk muscle demonstrating Dystrophin-YFP is targeted to the myoseptum. (G) The APEX-GBP single transgenic zebrafish fish demonstrates soluble reaction product. Arrows highlight the position of the myoseptum. (H) Electron micrograph of Dystrophin-YFP APEX-GBP double transgenic zebrafish muscle showing specific electron density at the myoseptum. Arrows highlight the position of the myoseptum and demonstrate reaction product accumulating at the site of the APEX-GBP modular tag and the Dystrophin-YFP protein in the double transgenic zebrafish line. CA, contractile apparatus; TT, transverse tubules; PM, plasma membrane. Scale bars represent 1 µm. |
Reprinted from Developmental Cell, 35(4), Ariotti, N., Hall, T.E., Rae, J., Ferguson, C., McMahon, K.A., Martel, N., Webb, R.E., Webb, R.I., Teasdale, R.D., Parton, R.G., Modular Detection of GFP-Labeled Proteins for Rapid Screening by Electron Microscopy in Cells and Organisms, 513-25, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell