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ZFIN ID: ZDB-FIG-151030-5
Antonio et al., 2015 - The wound inflammatory response exacerbates growth of pre-neoplastic cells and progression to cancer. The EMBO journal   34(17):2219-36 Full text @ EMBO J.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Term:
Stage Range: Long-pec to Day 5

Fig. 4

PGE2 is one component of the trophic signal driving woundinflammationtriggered preneoplastic cell proliferation

A–B′ Images of Ras+ larval flanks from control fish bathed in Danieau′s solution or with 10 µM NS398 to block Cox2 enzyme activity, and left unwounded (A, B) or laserwounded (A′, B′) at 3 dpf before subsequent fixation at 5 dpf.

C. Graph showing quantification of preneoplastic cell numbers at 5 dpf from (A–B′) (n = 15–20 larvae in each group).

D, D′ Unwounded control Ras+ larvae (D) versus sibling larvae that have been laserwounded at 2 dpf (D′).

E–F′ Unwounded versus wounded larvae after injection with PU1 and GCSF morpholinos (E and E′), and addition of 20 µM dmPGE2 (F and F′).

G. Graph showing preneoplastic cell numbers from (D–F′).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP hzm1Et; io006Tg control Long-pec trunk neoplasm IFL
Day 5 trunk neoplasm IFL
hzm1Et; io006Tg chemical treatment: NS-398 Day 5 trunk neoplasm IFL
hzm1Et; io006Tg resection: caudal fin Long-pec trunk neoplasm IFL
Day 5 trunk neoplasm IFL
hzm1Et; io006Tg laceration: trunk, chemical treatment: NS-398 Day 5 trunk neoplasm IFL
hzm1Et; io006Tg + MO1-spi1b + MO3-csf3r control Long-pec trunk neoplasm IFL
hzm1Et; io006Tg + MO1-spi1b + MO3-csf3r chemical treatment: prostaglandin E2 Long-pec trunk neoplasm IFL
hzm1Et; io006Tg + MO1-spi1b + MO3-csf3r resection: caudal fin Long-pec trunk neoplasm IFL
hzm1Et; io006Tg + MO1-spi1b + MO3-csf3r laceration: trunk, chemical treatment: prostaglandin E2 Long-pec trunk neoplasm IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal The EMBO journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ EMBO J.