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ZFIN ID: ZDB-FIG-151030-4
Antonio et al., 2015 - The wound inflammatory response exacerbates growth of pre-neoplastic cells and progression to cancer. The EMBO journal   34(17):2219-36 Full text @ EMBO J.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 3

Wounding leads to increased proliferation of pre-neoplastic cells.

A–D′ RasG12VeGFP larvae were left unwounded (A–D) or laserwounded (yellow dotted line) at 2 dpf just dorsal to the cloaca (A′–D′). Larvae were left to grow for 3 days before being fixed and analysed for preneoplastic cell number (n = 30 larvae in each group). EdU accumulation (purple) is shown at low magnification (C and C′) and a representative preneoplastic cell clone is shown (D and D′).

E. Graph illustrating preneoplastic cell numbers in unwounded versus wounded larvae (n = 27 of each group). Data from three independent experiments showed the same level of significance. ***P d 0.001.

F–G′ Images of larvae injected with control morpholino at the onecell stage, and either left unwounded (F, high magnification: G) or laserwounded at 3 dpf (F′, high magnification: G′) and fixed at 5 dpf.

H–J′ Larvae injected with a combination of PU1 and GCSF morpholinos at the onecell stage, left unwounded (H, high magnification: I) or laserwounded at 3 dpf (H′, high magnification: I′) and fixed at 5 dpf. Images of larvae injected with irf8 morpholino, and either unwounded (J) or laserwounded at 3 dpf (J′) before subsequent fixation at 5 dpf.

K, K′ Larvae injected with GCSF morpholino, and either left unwounded (K) or laserwounded at 3 dpf (K′) and fixed at 5 dpf.

L, M Graphs to show the total number of Ras+ cells and clones (respectively) at 5 dpf after PU1 and GCSF morpholinos.

N, O Graphs showing the number of Ras+ cells at 5 dpf after irf8 morpholino (N) or after GCSF morpholino injection (O).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 hzm1Et; io006Tg; nz50Tg control Day 5 neutrophil IHC
hzm1Et; io006Tg; nz50Tg resection: caudal fin Day 5 neutrophil IHC
hzm1Et; io006Tg; nz50Tg + MO1-irf8 control Day 5 neutrophil IHC
hzm1Et; io006Tg; nz50Tg + MO1-irf8 resection: caudal fin Day 5 neutrophil IHC
hzm1Et; io006Tg; nz50Tg + MO1-spi1b + MO3-csf3r control Day 5 neutrophil IHC
hzm1Et; io006Tg; nz50Tg + MO1-spi1b + MO3-csf3r resection: caudal fin Day 5 neutrophil IHC
hzm1Et; io006Tg; nz50Tg + MO3-csf3r control Day 5 neutrophil IHC
hzm1Et; io006Tg; nz50Tg + MO3-csf3r resection: caudal fin Day 5 neutrophil IHC
EGFP hzm1Et; io006Tg; nz50Tg control Day 5 trunk neoplasm IHC
hzm1Et; io006Tg; nz50Tg resection: caudal fin Day 5 trunk neoplasm IHC
hzm1Et; io006Tg; nz50Tg + MO1-irf8 control Day 5 trunk neoplasm IHC
hzm1Et; io006Tg; nz50Tg + MO1-irf8 resection: caudal fin Day 5 trunk neoplasm IHC
hzm1Et; io006Tg; nz50Tg + MO1-spi1b + MO3-csf3r control Day 5 trunk neoplasm IHC
hzm1Et; io006Tg; nz50Tg + MO1-spi1b + MO3-csf3r resection: caudal fin Day 5 trunk neoplasm IHC
hzm1Et; io006Tg; nz50Tg + MO3-csf3r control Day 5 trunk neoplasm IHC
hzm1Et; io006Tg; nz50Tg + MO3-csf3r resection: caudal fin Day 5 trunk neoplasm IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hzm1Et; io006Tg; nz50Tg resection: caudal fin Day 5 neutrophil increased amount, abnormal
hzm1Et; io006Tg; nz50Tg + MO1-irf8 control Day 5 macrophage absent, abnormal
hzm1Et; io006Tg; nz50Tg + MO1-irf8 resection: caudal fin Day 5 macrophage absent, abnormal
hzm1Et; io006Tg; nz50Tg + MO1-spi1b + MO3-csf3r control Day 5 macrophage absent, abnormal
Day 5 neutrophil absent, abnormal
hzm1Et; io006Tg; nz50Tg + MO1-spi1b + MO3-csf3r resection: caudal fin Day 5 macrophage absent, abnormal
Day 5 neutrophil absent, abnormal
hzm1Et; io006Tg; nz50Tg + MO3-csf3r control Day 5 neutrophil absent, abnormal
hzm1Et; io006Tg; nz50Tg + MO3-csf3r resection: caudal fin Day 5 neutrophil absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal The EMBO journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ EMBO J.