ZFIN ID: ZDB-FIG-151029-11
Mastracci et al., 2015 - Polyamine biosynthesis is critical for growth and differentiation of the pancreas. Scientific Reports   5:13269 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 7

Knockdown of dhps inhibits β cell differentiation.

(a) Representative images of neurod1:GFP + endocrine cells in the pancreas of control, 1 ng MO-injected, and 2 ng MO-injected Tg(neurod1:gfp) embryos at 72 hpf. Quantification of the number of (b) neurod1:GFP + cells in control (n = 15), 1 ng MO-injected (n = 15), and 2 ng MO-injected (n = 13) embryos (p = 0.0783), and (c) insulin + β cells in control (n = 8), 1 ng MO-injected (n = 8), and 2 ng MO-injected (n = 8) embryos (p = 0.012), at 72 hpf. (d) Representative images of the insulin + β cells from control, 1 ng MO-injected, and 2 ng MO-injected Tg(ptf1a:gfp) embryos at 72 hpf after label-retaining cell (LRC) assay was performed. LRC + cells express H2B-RFP. Quantification of the number of (e) insulin+/RFP + cells (LRC + or dorsal pancreas-derived cells) in control (n = 8), 1 ng MO-injected (n = 8), and 2 ng MO-injected (n = 8) embryos (p = 0.734), and (f) insulin+/RFP- cells (LRC- or ventral pancreas-derived cells) in control (n = 8), 1 ng MO-injected (n = 8), and 2 ng MO-injected (n = 8) embryos (p = 0.0147), at 72 hpf. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP jh1Tg control Protruding-mouth exocrine pancreas IFL
jh1Tg + MO1-dhps standard conditions Protruding-mouth exocrine pancreas IFL
nl1Tg standard conditions Protruding-mouth endocrine pancreas IFL
nl1Tg + MO1-dhps standard conditions Protruding-mouth endocrine pancreas IFL
ins jh1Tg standard conditions Protruding-mouth endocrine pancreas ISH
jh1Tg + MO1-dhps standard conditions Protruding-mouth endocrine pancreas ISH
nl1Tg standard conditions Protruding-mouth endocrine pancreas ISH
nl1Tg + MO1-dhps standard conditions Protruding-mouth endocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jh1Tg + MO1-dhps standard conditions Protruding-mouth endocrine pancreas ins expression decreased amount, abnormal
Protruding-mouth primary islet has fewer parts of type pancreatic B cell, abnormal
nl1Tg + MO1-dhps standard conditions Protruding-mouth anterior pancreatic bud type B pancreatic cell differentiation disrupted, abnormal
Protruding-mouth endocrine pancreas ins expression decreased amount, abnormal
Protruding-mouth primary islet has fewer parts of type pancreatic B cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.