ZFIN ID: ZDB-FIG-151029-8
Mastracci et al., 2015 - Polyamine biosynthesis is critical for growth and differentiation of the pancreas. Scientific Reports   5:13269 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. 4

Expression patterns of dhps and eif5a in zebrafish.

(a) Pathway illustrating the connection between the polyamine biosynthetic pathway, eIF5A, and DHS. Wholemount in situ hybridization for (b) dhps and (c) eif5a at 48 hpf and 72 hpf. At 72 hpf, higher magnification reveals expression of (d) dhps and (e) eif5a in the liver, intestine and pancreas. (f) Fluorescent in situ hybridization of eif5a in Tg(neurod1:gfp) transgenic fish at 72 hpf confirms expression in endoderm-derived organs, including swim bladder (SWB), intestine, liver, and pancreas.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dhps WT standard conditions Long-pec endoderm ISH
Long-pec pancreas ISH
Protruding-mouth intestine ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
EGFP nl1Tg control Protruding-mouth pancreas IFL
eif5a WT standard conditions Long-pec endoderm ISH
Long-pec pancreas ISH
Protruding-mouth intestine ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Protruding-mouth swim bladder ISH
nl1Tg standard conditions Protruding-mouth intestine ISH
Protruding-mouth liver ISH
Protruding-mouth swim bladder ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.