FIGURE

Fig. 2

ID

ZDB-FIG-150929-37

Publication

Rajaram et al., 2014 - Technical brief: Constant intense light exposure to lesion and initiate regeneration in normally pigmented zebrafish

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Fig. 2

Photoreceptor loss in pigmented and nonpigmented retinas. Pigmented tg:zop:nfsB:EGFP zebrafish (A) or nonpigmented albino zebrafish (B) were subjected to intense light damage, and retinas were collected at the indicated time points and processed for immunohistochemistry. Retinas in (A) were stained with zpr-1 (red) and anti-green fluorescent protein (GFP; green) antibodies, while retinas in (B) were stained with zpr-1 (red) antibody; nuclei were counter-stained with TOPRO (blue). Dorsal retinas are shown. Arrowheads indicate regions of photoreceptor loss. Arrows denote rod photoreceptors that have rounded up and lost their outer segments. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; OS, outer segments. Scale bar is 50 µm.

Expression Data

Gene:	EGFP
Antibody:	zpr-1
Fish:	slc45a2^b4/b4 nt19Tg
Condition:	high light intensity
Anatomical Terms:	green sensitive photoreceptor cell red sensitive photoreceptor cell retinal rod cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc45a2^b4/b4 nt19Tg
Condition:	high light intensity
Observed In:	green sensitive photoreceptor cell red sensitive photoreceptor cell retinal cone cell photoreceptor outer segment retinal outer nuclear layer retinal rod cell retinal rod cell photoreceptor outer segment
Stage:	Adult

Phenotype Detail

Acknowledgments

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