ZFIN ID: ZDB-FIG-150626-2
Xu et al., 2015 - Characterization of Tetratricopeptide Repeat-Containing Proteins Critical for Cilia Formation and Function. PLoS One   10:e0124378 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to Pec-fin

Fig. 4

Examinations on cilia in the Kupffer’s vesicle and pronephric duct.

(A-C) Both cilia number and cilia length in the Kupffer’s vesicle (KV) at the 7-somite stage were reduced in the indicated ttc morphants. Cilia were labeled using antibody against acetylated tubulin. The quantification results were based on three independent experiments. Student’s t-test, ***P < 0.001. Error bars represent s.d. (D) Typical morphology of cilia in pronephric ducts at 24 hpf. (E) and (F) Cilia motility in the pronephric duct at 60 hpf. The kymographs showed trajectories of the cilia marked with red line in S1, S2, S3, S4, S5 and S6 Videos. Cilia beat frequencies (CBFs) were measured at 60 hpf (40 cilia from 4 morphants for each gene). Student’s t-test, ***P < 0.001. Error bars represent s.e.m.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-odad4 standard conditions 5-9 somites Kupffer's vesicle cilium decreased amount, abnormal
5-9 somites Kupffer's vesicle cilium decreased length, abnormal
Prim-5 pronephric duct motile cilium disorganized, abnormal
Pec-fin cilium movement frequency, abnormal
WT + MO1-ttc36 standard conditions 5-9 somites Kupffer's vesicle cilium decreased amount, abnormal
5-9 somites Kupffer's vesicle cilium decreased length, abnormal
Prim-5 pronephric duct motile cilium disorganized, abnormal
Pec-fin cilium movement frequency, abnormal
WT + MO1-ttc39c standard conditions 5-9 somites Kupffer's vesicle cilium decreased amount, abnormal
5-9 somites Kupffer's vesicle cilium decreased length, abnormal
Prim-5 pronephric duct motile cilium disorganized, abnormal
Pec-fin cilium movement frequency, abnormal
WT + MO1-ttc4 standard conditions 5-9 somites Kupffer's vesicle cilium decreased amount, abnormal
5-9 somites Kupffer's vesicle cilium decreased length, abnormal
Prim-5 pronephric duct motile cilium disorganized, abnormal
Pec-fin cilium movement frequency, abnormal
WT + MO1-ttc9c standard conditions 5-9 somites Kupffer's vesicle cilium decreased amount, abnormal
5-9 somites Kupffer's vesicle cilium decreased length, abnormal
Prim-5 pronephric duct motile cilium disorganized, abnormal
Pec-fin cilium movement frequency, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One