ZFIN ID: ZDB-FIG-150519-17
Xu et al., 2015 - Characterization of Tetratricopeptide Repeat-Containing Proteins Critical for Cilia Formation and Function. PLoS One   10:e0124378 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3

Additional phenotypes of the zebrafish morphants of ttc4, -9c, -25, -36, and -39c.

(A) Typical otolith morphologies at 72 hpf. The otic vesicle normally contains two otoliths as shown. Otoliths that show differences in number, size and/or position were considered as abnormal. (B) Quantification results for abnormal otoliths in the indicated morphants. (C) and (D) Hydrocephalus formation (arrows in the bright-field images and asterisks in the histochemical sections) in the indicated morphants at 72 hpf. (E) and (F) The ttc25 and ttc4 morphants tended to develop pronephric cysts (arrows in the bright-field images and asterisks in the histochemical sections) at 72 hpf. (G) and (H) Left-right asymmetry patterning was disturbed in the indicated ttc morphants at 30 hpf. The cmlc2 probe was used to label the heart tube (arrows) in whole-mount in situ hybridization. All the quantification results were based on three independent experiments. Student’s t-test, ***P < 0.001. Error bars represent s.d.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-odad4 standard conditions Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth determination of heart left/right asymmetry disrupted, abnormal
Protruding-mouth otolith morphology, abnormal
Protruding-mouth pronephros cystic, abnormal
Protruding-mouth ventricular system dilated, abnormal
WT + MO1-ttc36 standard conditions Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth determination of heart left/right asymmetry disrupted, abnormal
Protruding-mouth otolith morphology, abnormal
Protruding-mouth pronephros morphology, normal
Protruding-mouth ventricular system dilated, abnormal
WT + MO1-ttc39c standard conditions Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth determination of heart left/right asymmetry disrupted, abnormal
Protruding-mouth otolith morphology, abnormal
Protruding-mouth pronephros morphology, normal
Protruding-mouth ventricular system dilated, abnormal
WT + MO1-ttc4 standard conditions Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth determination of heart left/right asymmetry disrupted, abnormal
Protruding-mouth otolith morphology, abnormal
Protruding-mouth pronephros cystic, abnormal
Protruding-mouth ventricular system dilated, abnormal
WT + MO1-ttc9c standard conditions Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth determination of heart left/right asymmetry disrupted, abnormal
Protruding-mouth otolith morphology, abnormal
Protruding-mouth pronephros morphology, normal
Protruding-mouth ventricular system dilated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One