Screen for cilia-related TTC genes in zebrafish.
(A) The morphologies of zebrafish morphants at 72 hpf. Note that the morphants of ttc4, -9c, -25, -36, and -39c displayed severe body curving. (B) Quantification results for the curved body phenotype. Three independent experiments were performed. (C) and (D) Rescue experiments for the ttc morphants. The indicated MO was coinjected with either the corresponding MO-resistant mRNA or GFP mRNA into zebrafish embryos. The body curvature phenotype was assayed at 72 hpf. The numbers of embryos counted are listed over the histograms. Statistical results are from three independent experiments. Student’s t-test, ***P < 0.001. Error bars represent s.d.