ZFIN ID: ZDB-FIG-150519-16
Xu et al., 2015 - Characterization of Tetratricopeptide Repeat-Containing Proteins Critical for Cilia Formation and Function. PLoS One   10:e0124378 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 2

Screen for cilia-related TTC genes in zebrafish.

(A) The morphologies of zebrafish morphants at 72 hpf. Note that the morphants of ttc4, -9c, -25, -36, and -39c displayed severe body curving. (B) Quantification results for the curved body phenotype. Three independent experiments were performed. (C) and (D) Rescue experiments for the ttc morphants. The indicated MO was coinjected with either the corresponding MO-resistant mRNA or GFP mRNA into zebrafish embryos. The body curvature phenotype was assayed at 72 hpf. The numbers of embryos counted are listed over the histograms. Statistical results are from three independent experiments. Student’s t-test, ***P < 0.001. Error bars represent s.d.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-odad4 standard conditions Protruding-mouth trunk curved, abnormal
WT + MO1-ttc36 standard conditions Protruding-mouth trunk curved, abnormal
WT + MO1-ttc39c standard conditions Protruding-mouth trunk curved, abnormal
WT + MO1-ttc4 standard conditions Protruding-mouth trunk curved, abnormal
WT + MO1-ttc9c standard conditions Protruding-mouth trunk curved, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One