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ZFIN ID: ZDB-FIG-150601-4
Gutierrez-Triana et al., 2014 - A vertebrate-conserved cis -regulatory module for targeted expression in the main hypothalamic regulatory region for the stress response. BMC Developmental Biology   14:41 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Fig. 4

NPO cells involved in the HPA axis are labeled in theTg(otpECR6-E1b:mmGFP)transgenic line. A, There is no overlap of GFP with in situ stained cck+ cells. B, There is some overlap of GFP with penka+ cells. C, There is extensive overlap of GFP with oxt+ cells. D, There is no overlap of GFP with vip+ cells. E, There is a high degree of overlap of GFP with avp+ cells. F, There is some overlap of GFP with sst1.1+ cells. G, There is a high degree of overlap of GFP with crh + cells. H, There is some overlap of GFP with nts+ cells. I, There is no overlap of GFP with penkb + cells. J, There is no overlap of GFP with immunostained TH+ cells. Abbreviations: r, rostral; l, lateral. Scale bars: 50 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
avp hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
ccka hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
crhb hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
GFP hd12Tg standard conditions Protruding-mouth to Day 5 Not Detected diencephalon dopaminergic neuron IHC
Protruding-mouth to Day 5 preoptic area IHC
Protruding-mouth to Day 5 preoptic area neurosecretory neuron IHC
nts hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
oxt hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
penka hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
penkb hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
sst1.1 hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
vip hd12Tg standard conditions Protruding-mouth to Day 5 preoptic area ISH
Protruding-mouth to Day 5 preoptic area neurosecretory neuron ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab6-th IHC hd12Tg standard conditions Protruding-mouth - Day 5 diencephalon dopaminergic neuron
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.