ZFIN ID: ZDB-FIG-150526-2
Ye et al., 2015 - glucagon is essential for alpha cell transdifferentiation and beta cell neogenesis. Development (Cambridge, England)   142:1407-17 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Day 5 to Day 6

Fig. 2

δ cells, but not regenerating β cells, are lineage marked by sst2:Cre. (A-A′′′) Merged and single-channel images of 5-dpf Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets that were heat shocked at 3dpf and stained for GFP (green), insulin (red) and somatostatin (white). Somatostatin+ δ cells are labeled by H2B-GFP. (B-C′′′) Merged and single-channel images of 5-dpf non-ablated (B) or 1dpa (C) Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets that were heat shocked at 3dpf and stained for GFP (green), insulin (red) and DNA (blue). (D) Quantification of total somatostatin+, somatostatin+/H2B-GFP+, insulin+, and insulin+/H2B-GFP+ cells in non-ablated 5-dpf Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets. 45% of somatostatin+ cells and 1.5% of insulin+ cells are marked by sst2:Cre (n=15). (E) Quantification of insulin+ and insulin+/H2B-GFP+ cells in 5-dpf regenerating islets. 1% of insulin+ cells are marked by sst2:Cre (n=9).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s923Tg; s950Tg; s963Tg heat shock Day 5 pancreatic B cell IHC
s923Tg; s950Tg; s963Tg heat shock, chemical treatment: pharmaceutical Day 6 pancreatic B cell IHC
ins s923Tg; s950Tg; s963Tg heat shock Day 5 pancreatic B cell IHC
s923Tg; s950Tg; s963Tg heat shock, chemical treatment: pharmaceutical Day 6 pancreatic B cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
ab2-sst IHC s923Tg; s950Tg; s963Tg heat shock Day 5 pancreatic D cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development