ZFIN ID: ZDB-FIG-150526-1
Ye et al., 2015 - glucagon is essential for alpha cell transdifferentiation and beta cell neogenesis. Development (Cambridge, England)   142:1407-17 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Day 4 to Days 14-20

Fig. 1

β cell neogenesis from α cell transdifferentiation in zebrafish. (A-E) Confocal projections showing α (red) and β (green) cells in the principal islet of intact (A,B) and ablated (C-E) Tg(ins:CFP-NTR) larvae at 0, 1 and 16days post ablation (dpa). Scale bars: 10 ┬Ám. (F) Quantification of insulin+ cells in intact (gray line) and regenerating (red line) islets from 0 to 20dpf (ne3 for all groups except control 20dpf, n=1). The ratio of β cells in regenerating versus control islets is indicated. (G) Blood glucose measurements of non-ablated larvae (gray) and larvae in which β cells were ablated from 3-4dpf (red) (n=3). (H,I) Confocal projections of β cell lineage-marked islets in 5-dpf control and 1-dpa Tg(ins:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) larvae. β cells were labeled by inducible H2B-GFP at 3dpf before ablation and stained for GFP (green) and insulin (red). (J,K) Confocal planes of ablated (J) and regenerating (K) islets in Tg(ins:Flag-NTR); Tg(gcga:GFP); Tg(ins:dsRed) larvae. Red arrow in K indicates gcga:GFP+ ins:dsRed+ β cells in regenerating islet. (L-N) Confocal planes of Tg(gcga:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets labeled by H2B-GFP before ablation, and stained for GFP (green), insulin (red) and glucagon (blue). α cells are indicated by white arrows and β cells by the red arrow. (L) 6-dpf non-ablated islet, (M) 4-dpf ablated islet at 0dpa, and (N) 6-dpf islet at 2dpa. H2B-GFP+ regenerating β cells are indicated with yellow arrows. (O) Quantification of H2B-GFP+ and H2B-GFP β cells in 2-dpa islets (n=10). ***P≤0.001 (Student′s t-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed ia1Tg; m1018Tg; s950Tg chemical treatment: pharmaceutical Day 4 Not Detected pancreatic B cell IFL
Day 6 pancreatic B cell IFL
GFP ia1Tg; m1018Tg; s950Tg chemical treatment: pharmaceutical Day 4 pancreatic A cell IFL
Day 6 pancreatic A cell IFL
s923Tg; s924Tg; s950Tg control Day 5 pancreatic B cell IFL
s923Tg; s924Tg; s950Tg heat shock, chemical treatment: pharmaceutical Day 5 Not Detected pancreatic B cell IFL
s923Tg; s950Tg; s962Tg control Day 6 pancreatic B cell IHC
s923Tg; s950Tg; s962Tg heat shock, chemical treatment: pharmaceutical Day 4 pancreatic B cell IHC
Day 6 pancreatic B cell IHC
ins WT control Day 4 pancreatic B cell IHC
Days 14-20 pancreatic B cell IHC
WT chemical treatment: pharmaceutical Day 4 Not Detected pancreatic B cell IHC
Day 5 pancreatic B cell IHC
Days 14-20 pancreatic B cell IHC
s923Tg; s924Tg; s950Tg control Day 5 pancreatic B cell IHC
s923Tg; s924Tg; s950Tg heat shock, chemical treatment: pharmaceutical Day 5 pancreatic B cell IHC
s923Tg; s950Tg; s962Tg control Day 6 pancreatic B cell IHC
s923Tg; s950Tg; s962Tg heat shock, chemical treatment: pharmaceutical Day 4 Not Detected pancreatic B cell IHC
Day 6 pancreatic B cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gcg IHC WT control Day 4 pancreatic A cell
IHC Days 14-20 pancreatic A cell
IHC WT chemical treatment: pharmaceutical Day 4 pancreatic A cell
IHC Day 5 pancreatic A cell
IHC Days 14-20 pancreatic A cell
IHC s923Tg; s950Tg; s962Tg control Day 6 pancreatic A cell
IHC s923Tg; s950Tg; s962Tg heat shock, chemical treatment: pharmaceutical Day 4 pancreatic A cell
IHC Day 6 pancreatic A cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development