Fig. 6

Rescue of apoptosis with the wild-type body extract. (A) Procedure of tail explant culture. Following fin fold amputation, the tail tissue was isolated and placed in the culture medium. At 12 hpa, acridine orange staining was performed to detect apoptotic cells. (B) Reproduction of the blastema apoptosis phenotype in the tail explant. Blastema apoptosis was detected in the mutant explant, but not in the wild-type one. (C) Detection of TUNEL-positive cells in the tnnt2a/silent heart morphant. Although almost all morphants did not have the heartbeat, no significant induction of apoptosis was observed. n=50 (tnnt2a MO) and 17 (standard control MO), respectively. (D) Quantification of the number of TUNEL-positive cells (brackets) in (C). (E) Rescue of the apoptosis in the tal1 mutant with the wild-type extract, but not the mutant one. Similar results were obtained more than three independent experiments. (F) Quantification of the number of TUNEL-positive cells in the explant culture. n=15 (tal1), 14 (WT), 13 (tal1+WT body fluid), and 14 (tal1+tal1 body fluid), respectively. Data in (D) and (F) are presented as mean±s.e.m. Statistical significance was tested using Welchós t test. **P<0.001. N.S., not significant. The dotted lines in (B), (C) and (E) indicate the outline of the fin fold. Scale bars represent 100 µm.