ZFIN ID: ZDB-PUB-141224-20
A diffusible signal derived from hematopoietic cells supports the survival and proliferation of regenerative cells during zebrafish fin fold regeneration
Hasegawa, T., Nakajima, T., Ishida, T., Kudo, A., Kawakami, A.
Date: 2015
Source: Developmental Biology   399(1): 80-90 (Journal)
Registered Authors: Kawakami, Atsushi, Kudo, Akira
Keywords: Apoptosis, Fin fold, Myeloid, Regeneration, Zebrafish, cloche, tal1
MeSH Terms:
  • Animal Fins/injuries
  • Animal Fins/physiology*
  • Animals
  • Animals, Genetically Modified
  • Apoptosis/genetics
  • Apoptosis/physiology
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Cell Proliferation*
  • Cell Survival/genetics
  • Cell Survival/physiology
  • Hematopoietic Stem Cells/metabolism*
  • In Situ Hybridization
  • Larva/cytology
  • Larva/metabolism
  • Larva/physiology
  • Microscopy, Confocal
  • Mutation
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism
  • Regeneration/genetics
  • Regeneration/physiology*
  • Signal Transduction/genetics
  • Signal Transduction/physiology*
  • Wound Healing/genetics
  • Wound Healing/physiology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 25533245 Full text @ Dev. Biol.
Multicellular organisms maintain body integrity by constantly regenerating tissues throughout their lives; however, the overall mechanism for regulating regeneration remains an open question. Studies of limb and fin regeneration in teleost fish and urodeles have shown the involvement of a number of locally activated signals at the wounded site during regeneration. Here, we demonstrate that a diffusible signal from a distance also play an essential role for regeneration. Among a number of zebrafish mutants, we found that the zebrafish cloche (clo) and tal1 mutants, which lack most hematopoietic tissues, displayed a unique regeneration defect accompanying apoptosis in primed regenerative tissue. Our analyses of the mutants showed that the cells in the primed regenerative tissue are susceptible to apoptosis, but their survival is normally supported by the presence of hematopoietic tissues, mainly the myeloid cells. We further showed that a diffusible factor in the wild-type body fluid mediates this signal. Thus, our study revealed a novel mechanism that the hematopoietic tissues regulate tissue regeneration through a diffusible signal.