Fig. 3

Cell death in the clo mutant is the apoptosis of primed regenerative cells. (A) TUNEL analysis during fin fold regeneration in the clo larvae that was injected or uninjected with bcl2 mRNA. clo-specific cell death (brackets) was significantly suppressed by Bcl2 overexpression. (B) Quantification of TUNEL-positive cells in (A). n=12 (injected) and 8 (uninjected), respectively. Data are presented as mean±s.e.m. Statistical significance was tested using Welchós t test. **P<0.001. (C) TUNEL analysis in the double mutants of clo and tp53. Apoptosis in the clo mutant (brackets) was not affected by the absence of tp53. (D) Quantification of the number of TUNEL-positive cells in (C). n=14 (clo^-/-; tp 53^-/-) and 27 (clo^-/--; tp53^+/?), respectively. (E) ISH analysis of junb and junbl, markers of the wound epidermis and blastema, respectively. Both markers were expressed in the clo mutant at higher levels than wild-type (n=7/7, respectively). (F) Horizontal cross section of clo fin fold that were made after TUNEL staining. Predominant localization of TUNEL-positive cells in the mesenchymal region in the clo mutant. The dotted lines in (A) and (C) indicate the outlines of fin folds. Scale bars represent 100 µm in (A) and (C), and 50 µm in (E) and (F).