Fig. 4

Sox4 knockdown causes ectopic cell proliferation in the retina. (A) Quantification of PH3-positive cells in control and sox4 morphant retinas from 18 to 72 hpf. 8–10 individuals were analyzed in each time point (Studentós t-test, P<0.01). (B–K) Representative transverse sections through the optic vesicle and retina of control and sox4 morphants immunolabeled for PH3 at the indicated time points. Ectopic PH3-positive cells are indicated by arrows. At 72 hpf, the ectopic PH3-positive cells in the GCL also clustered with PCNA-positive cells (K, arrow). (L) Quantification of TUNEL-positive cells in control and sox4 morphant optic vesicle and retina from 18 to 72 hpf. 10–20 individuals were analyzed for each time point (Studentós t-test, P=0.017). (M–V) Representative transverse sections of control and sox4 morphant retinas labeled for TUNEL-positive cells from 18 to 72 hpf. (W) Quantification of TUNEL-positive cells in control and sox4 morphant optic stalk at 18 hpf. Arrows in N indicate increased TUNEL-positive cells in the optic stalk of an 18 hpf sox4 morphant. All scale bars equal 50 µm.