header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150326-58
Saito et al., 2014 - Telomere distribution pattern and synapsis initiation during spermatogenesis in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   243(11):1448-56 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Adult

Fig. 8

Analysis of the distribution of telomeres by performing FISH using a telomere probe in combination with immunocytochemical labeling of Sycp3 in spermatocytes of the imo zebrafish mutant. FISH and labeling of Sycp3 were performed in the imo spermatocytes at leptotene (A), early zygotene (B), and zygotene (C) stages. A: Telomeres localize within a small region of the nuclear periphery and form a bouquet configuration at the leptotene stage. B: At the early zygotene stage, telomeres start to move over the nuclear periphery. C: Telomeres disperse over the nuclear periphery at the zygotene stage. Left panels show Sycp3 staining (green), middle panels show FISH signals (magenta), and right panels show merged images of the left and middle panels. Nuclei were stained with TO-PRO-3 (blue). Scale bar = 10 ┬Ám. These images are representative of 20 nuclei analyzed at each stage per specimen (n = 3).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sycp3 Ab1-sycp3 imot30312/t30312 standard conditions Adult spermatocyte nucleus IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.