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ZFIN ID: ZDB-FIG-150326-53
Saito et al., 2014 - Telomere distribution pattern and synapsis initiation during spermatogenesis in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   243(11):1448-56 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 3

Distribution and assembly of telomeres in zebrafish spermatogenic cells. A–C: Telomeres in spermatogonia. Spermatogonia were identified by EGFP expression in vas::EGFP transgenic zebrafish testis. Panel A shows anti-GFP antibody staining (green), panel B shows FISH signals using a telomere probe (magenta), and panel C shows a merged image of panels A and B. D–L: Telomeres in meiotic spermatocytes. Spermatocytes at preleptotene (DF), leptotene or early zygotene (GI), and pachytene (JL) stages were identified according to their Sycp3 expression patterns. Panels D, G, and J show anti-Sycp3 antibody staining (green); panels E, H, and K show FISH signals using a telomere probe (magenta); and panels F, I, and L show merged images. Nuclei were stained with TO-PRO-3 and are displayed in blue (AL). M–R: Distributions of telomeres in spermatids (MO) and sperm (PR). Panels M and P show nuclear staining with TO-PRO-3, panels N and Q show FISH signals using a telomere probe, and panels O and R show merged images (TO-PRO-3, green; FISH signal, magenta). Scale bar = 10 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf45Tg standard conditions Adult spermatogonium IFL
sycp3 Ab1-sycp3 zf45Tg standard conditions Adult spermatocyte IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.