ZFIN ID: ZDB-FIG-150318-49
Solin et al., 2014 - Molecular and Cellular Characterization of a Zebrafish Optic Pathway Tumor Line Implicates Glia-Derived Progenitors in Tumorigenesis. PLoS One   9:e114888 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4 Characterization of lincRNAis18 tumors indicates a glial cell origin.

Immunolabeling and in situ hybridization of cryosections from wild type retina (A–D, I, J, M–P) and advanced lincRNAis18 tumors (E–H, K, L, Q–T). Cells in rosettes in the tumors label with neurofilament marker RT-97 (red) and photoreceptor marker recoverin (green) (E, F). The synaptic vesicle marker SV2 (green), which is enriched in the retinal plexiform layers (C), was distributed throughout the fibrous tumor mass (G). (D) BrdU (green) incorporated into proliferating progenitor cells at the ciliary marginal zone of the wild type retina (arrow). (H) Intense labeling of BrdU incorporation was detected in cells forming rosettes and throughout the tumor mass. In wild type the glial marker GFAP is most evident in the Müller glia end feet that sit in the retinal ganglion cell layer (I–L). GFAP expression is absent in the oligodendrocytes and astrocytes of the optic nerve (I, K arrow). In contrast, GFAP was readily detected in long streaks throughout the tumor tissue (M, N, P), and was expressed by cells in the mutant optic nerve (O, arrow). BLBP is present at the ciliary marginal zone of wild type retina (L) and appeared present throughout the tumor mass (P). A, E, I, M, Differential interference contrast (DIC) overlay on immunofluorescence labeling images. CMZ, ciliary marginal zone; GCL, ganglion cell layer; INL, inner nuclear layer; ON, optic nerve; ONH, optic nerve head; ONL, outer nuclear layer. All scale bars represent 50 µm, except in panels K and O scale bars represent 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gfap zrf-1 WIK standard conditions Adult Not Detected cranial nerve II glial cell IHC
zrf-1 Adult Muller cell glial limiting end-foot IHC
zrf-1 si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult cranial nerve II IHC
zrf-1 Adult retina glioma IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC WIK standard conditions Adult retina synaptic vesicle
IHC si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult eye neoplasm
Ab1-RT97 IHC WIK standard conditions Adult photoreceptor outer segment layer
IHC Adult retina intermediate filament
IHC si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult eye neoplasm
Ab2-recoverin IHC WIK standard conditions Adult retinal photoreceptor layer
IHC si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult eye neoplasm
Ab3-fabp7 IHC WIK standard conditions Adult ciliary marginal zone
IHC si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult eye neoplasm
Phenotype Details
Fish Conditions Stage Phenotype
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult cranial nerve II glioma present, abnormal
Adult eye neoplasm proliferative, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One