ZFIN ID: ZDB-FIG-150212-8
Ceci et al., 2014 - Axon-Schwann cell interactions during peripheral nerve regeneration in zebrafish larvae. Neural Development   9:22 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Terms:
Stage: Day 5
PHENOTYPE:
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Observed In:
Stage: Day 5

Fig. 8 pLL nerve regeneration in larvae totally or partially lacking Schwann cells. Transgenic tg(neuroD:EGFP) or tg(NeuroD:TagRFP) were used to evaluate the impact of Schwann cell absence on pLL nerve regeneration. (A) After 48 hpn, the regrowing nerve of control but neurectomized larvae reaches the tip of the tail. (B-F) Transgenic larvae were treated with AG1478 following two treatments: 5 µM AG1478 from 10 to 58 hpf (B-D) and 3.5 µM AG1478 from 24 to 58 hpf (E, F). In both cases, the drug was washed out at 58 hpf and the larvae were maintained in E3 medium. At 3 dpf, larvae of each treatment were separated into two groups: non-neurectomized AG-treated controls and neurectomized AG-treated larvae. Note that the nerve of control (and treated) larvae deviates from the myoseptum (dotted line) in both treatments (B, E) but deviation is more severe in the longer treatment; insets show larger magnification. In parallel, the pLL nerve of neurectomized larvae fails to regenerate properly as it defasciculates and wanders outside of the horizontal myoseptum (C, D, F), and fails to reach the tip of the tail at 48 hpn under these conditions. (G,H) At 5 dpf we quantified and compared the winding index of the nerve in neurectomized vs. non-neurectomized larvae. In both treatments, the pLL axons of neurectomized larvae have an increased winding index in comparison with their respective controls. ***P <0.001. Scale: A-F: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg control Day 5 posterior lateral line nerve IFL
Day 5 posterior lateral line nerve axon IFL
nl1Tg ablation: posterior lateral line nerve, chemical treatment: tyrphostin AG 1478 Day 5 posterior lateral line nerve IFL
Day 5 posterior lateral line nerve axon IFL
RFP w69Tg control Day 5 posterior lateral line nerve IFL
Day 5 posterior lateral line nerve axon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg ablation: posterior lateral line nerve, chemical treatment: tyrphostin AG 1478 Day 5 myelinating Schwann cell absent, abnormal
Day 5 posterior lateral line nerve defasciculated, abnormal
Day 5 posterior lateral line nerve axon guidance process quality, abnormal
Day 5 posterior lateral line nerve axon regeneration process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.