ZFIN ID: ZDB-FIG-150212-10
Ceci et al., 2014 - Axon-Schwann cell interactions during peripheral nerve regeneration in zebrafish larvae. Neural Development   9:22 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 10 Role of ErbB signaling in axonal regeneration. Three days post fertilization larvae were neurectomized (circles indicate position of neurectomy) after incubation with 0.05% of DMSO (controls) or 5 µM of AG1478 from 60 hpf to 5 dpf (48 hpn); this treatment does not affect Schwann cell survival but blocks ErbB signaling during the regeneration phase. Nerve regeneration in control (A-C) and drug treated larvae (D-F) was evaluated after 10 hpn, 14 hpn, and 48 hpn. The insets in C and F show the defasciculation of the regenerated nerve in treated larvae in comparison to controls. (G-I) Quantitative analysis of axonal extension after 10 hpn (G), 14 hpn (H), and 48 hpn (I) in control and AG1478 treated larvae. *P <0.05; ***P <0.001. (J) In 30% of ErbB inhibitor treated larvae, the non-neurectomized contralateral nerve appears defasciculated in comparison to the control nerve. Scale bar: C, F: 200 µm; A, B, D, E, J; insets in C and F: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg ablation: posterior lateral line nerve, chemical treatment: tyrphostin AG 1478 Protruding-mouth posterior lateral line nerve IFL
Protruding-mouth posterior lateral line nerve axon IFL
Day 5 posterior lateral line nerve IFL
Day 5 posterior lateral line nerve axon IFL
nl1Tg ablation: posterior lateral line nerve Protruding-mouth posterior lateral line nerve IFL
Protruding-mouth posterior lateral line nerve axon IFL
Day 5 posterior lateral line nerve IFL
Day 5 posterior lateral line nerve axon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg ablation: posterior lateral line nerve, chemical treatment: tyrphostin AG 1478 Protruding-mouth posterior lateral line nerve defasciculated, abnormal
Protruding-mouth posterior lateral line nerve axon guidance process quality, abnormal
Protruding-mouth posterior lateral line nerve axon regeneration delayed, abnormal
Protruding-mouth posterior lateral line nerve axon regeneration process quality, abnormal
Day 5 posterior lateral line nerve defasciculated, abnormal
Day 5 posterior lateral line nerve axon guidance process quality, abnormal
Day 5 posterior lateral line nerve axon regeneration delayed, abnormal
Day 5 posterior lateral line nerve axon regeneration process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.